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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (PhD) (INTERDISCIPLINARY) | |
| Code | BT009 |
| Name | Molecular Plant Breeding |
| Term | 2018-2019 Academic Year |
| Term | Fall |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
Giving information about introduction of molecular genetics laboratory, DNA isolation in plants, determination of DNA quantity and quality, electrophoresis systems, PCR applications, cloning of DNA
Course Content
Introduction to molecular genetics laboratory, DNA isolation in plants, determination of DNA quantity and quality, electrophoresis systems, PCR applications, cloning of DNA
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications |
| LO02 | Understanding the molecular techniques and the interaction between the related disciplines |
| LO03 | Solving problems by research methods and establishing cause-effect relationship |
| LO04 | To be able to understand the studies in molecular biology laboratory |
| LO05 | Learn about the solutions used in the laboratory |
| LO06 | Understanding of DNA isolation |
| LO07 | PCR and electrophoresis applications |
| LO08 | Mid-Term Exam |
| LO09 | Understanding biochemical markers |
| LO10 | Understanding hybridization based markers |
| LO11 | Understanding PCR based markers (RAPD, ISSR) |
| LO12 | Understanding PCR based markers (SSR, SRAP) |
| LO13 | Understanding cDNA AFLP method |
| LO14 | Understanding Microarray method |
| LO15 | To be able to understand similarity index, creation and evaluation of dendograms |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | Develop and deepen the current and advanced knowledge in the field with original thought and / or research at the level of expertise based on the qualifications of the graduate and reach the original definitions that will bring innovation to the field. | |
| PLO02 | - | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | |
| PLO03 | - | Evaluates, uses and transfers new information in the field with a systematic approach. | |
| PLO04 | - | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | |
| PLO05 | - | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | |
| PLO06 | - | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | |
| PLO07 | - | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | |
| PLO08 | - | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO09 | - | Conducts scientific research in national and international scientific research groups. | |
| PLO10 | - | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to molecular genetics laboratory, preparation of solution | Reading the related lecture notes | |
| 2 | DNA isolation in plants | Reading the related lecture notes | |
| 3 | Determination of DNA quality and quantity | Reading the related lecture notes | |
| 4 | Polymerase chain reaction (PCR) | Reading the related lecture notes | |
| 5 | Preparation of agarose gel and polyacrylamide gel | Reading the related lecture notes | |
| 6 | Biochemical markers and their use in breeding | Reading the related lecture notes | |
| 7 | Molecular techniques and applications based on hybridization (RFLP) | Reading the related lecture notes | |
| 8 | Mid-Term Exam | Preparation of the exam | |
| 9 | PCR based molecular techniques and applications (RAPD, ISSR) | Reading the related lecture notes | |
| 10 | PCR based molecular techniques and applications (SSR, SRAP) | Reading the related lecture notes | |
| 11 | PCR based molecular techniques and applications (AFLP, CAPs) | Reading the related lecture notes | |
| 12 | Determination of gene expression (cDNA AFLP) | Reading the related lecture notes | |
| 13 | Determination of gene expression (Microarray) | Reading the related lecture notes | |
| 14 | Determination of gene expression as quantitative (Real Time PCR) | Reading the related lecture notes | |
| 15 | Similarity index, creation and evaluation of dendograms | Reading the related lecture notes | |
| 16 | Term Exams | Preparation of the exam | |
| 17 | Term Exams | Preparation of the exam |