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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (PhD) (INTERDISCIPLINARY) | |
| Code | BT553 |
| Name | Analysis of transgenic plants and propagation materials in Genetically modified organisms |
| Term | 2018-2019 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MUKADDES KAYIM |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
Giving information about genetically modified plant production techniques and their contents in the world. To teach how to do analysis of such plants and production materials.
Course Content
Trangenic plant production methods will be explained in detail. The characteristics of commercial transgenic products will be discussed, the analysis methods will be explained in detail and will be shown in practice.
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Gains general information about genetically modified organisms |
| LO02 | Learn gene transfer techniques |
| LO03 | Learn about the use of transgenic plants in agriculture |
| LO04 | Develops an opinion on the positive and negative effects of transgenic plants on the environment |
| LO05 | Learns that transgenic plants can be analyzed by different methods |
| LO06 | Learn the replication of transgenes with polymerase chain reaction in the analysis of transgenic plants |
| LO07 | Learns to detect transgenic protein by Western blot from serological methods in the analysis of transgenic plants |
| LO08 | Learns how to clone target genes in transgenic plant acquisition |
| LO09 | Gains knowledge of where the marker genes are obtained from which biotic organisms are found on the vector in transgenic plant production |
| LO10 | Learns the functions of promoter and terminator genes in transgenic plant production and how to use them in GMO analysis |
| LO11 | Learns which methods and genes are used in GMO analysis in Turkish Standards Institute |
| LO12 | Learn about the genes and analysis used in GMO analysis in the world |
| LO13 | Learns which methods are more sensitive, more practical or expensive in GMO analysis |
| LO14 | Learns which methods are used in practice in GMO analysis laboratories |
| LO15 | Learn to read GMO analysis reports |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develop and deepen the current and advanced knowledge in the field with original thought and / or research at the level of expertise based on the qualifications of the graduate and reach the original definitions that will bring innovation to the field. | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | 1 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | 3 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | 4 |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. | 2 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Techniques used in gene transfer | Related articles, books and lecture notes | |
| 2 | Basic principles of Agrobacterium-mediated gene transfer | Related articles, books and lecture notes | |
| 3 | Methods for direct gene transfer | Related articles, books and lecture notes | |
| 4 | Properties of plasmid vectors used in gene transfer | Related articles, books and lecture notes | |
| 5 | Marker genes used in cloning target gene cassettes | Related articles, books and lecture notes | |
| 6 | Properties of marker genes | Related articles, books and lecture notes | |
| 7 | Introduction to methods used in the analysis of genetically modified plants | Related articles, books and lecture notes | |
| 8 | Mid-Term Exam | Written exam | |
| 9 | Promoter genes and their analysis | Related articles, books and lecture notes | |
| 10 | Terminator genes and their analysis | Related articles, books and lecture notes | |
| 11 | Southern, Northern and Western blotting techniques | Related articles, books and lecture notes | |
| 12 | GMO analysis genes used by Turkish Standards Institute | Related articles, books and lecture notes | |
| 13 | Homework presentation and performance evaluation | Related articles | |
| 14 | Transgenic plant analysis in articles | Related articles, books and lecture notes | |
| 15 | Homework presentation and performance evaluation | Related articles | |
| 16 | Term Exams | Written exam | |
| 17 | Term Exams | Written exam |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 5 | 70 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 15 | 15 |
| Total Workload (Hour) | 160 | ||
| Total Workload / 25 (h) | 6,40 | ||
| ECTS | 6 ECTS | ||