TÜRKÇE
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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| HORTICULTURAL CROPS (MASTER) (WITH THESIS) | |
| Code | BB030 |
| Name | Using of Molecular Techniques in Hoticultural Plants |
| Term | 2020-2021 Academic Year |
| Term | Spring |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
Information about DNA,RNA and PCR, types of molecular markers, advantages / disadvantages and usage areas of horticulture breeding
Course Content
Information about DNA, RNA and PCR, solution preparation, types of molecular markers (RAPD, SSR, ISSR etc.), advantages / disadvantages of molecular markers and their use in plant breeding
Course Precondition
Resources
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Developing and improving the horticultural knowledge to professional level based on the bachelors degree qualifications |
| LO02 | Understanding the horticulture and the interaction between the related disciplines |
| LO03 | Solving problems by research methods and establishing cause-effect relationship |
| LO04 | Using the theoretical and practical knowledge of Horticulture and Molecular Biology, supporting the related up to date progress with the quantitative and qualitative data, combining and interpreting the information and data obtained from different disciplines and developing new information and theories by synthesis |
| LO05 | Carrying out a study individually that requires professional knowledge in Horticulture and Molecular Biology |
| LO06 | Analytically approaching in order to solve unpredictable and complex problems encountered in applications related to Horticulture and Molecular Biology, planning the research process, producing solutions by taking responsibility and evaluating-defending the obtained results |
| LO07 | Leading in the situations when solving problems related to breeding of Horticulture is necessary |
| LO08 | Evaluation of knowledge via midterm exam |
| LO09 | Entering database related to breeding of horticulture, utilizing those sources and self-updating. |
| LO10 | Evaluating the knowledge and skills in breeding of horticulture by criticism and directing their own learning process |
| LO11 | Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments |
| LO12 | Using software related to horticulture and molecular markers sufficiently and using information and communication technologies in advanced level |
| LO13 | Obtaining data by scientific methods in order to solve a problem related to horticulture molecular breeding, establishing and interpreting the data by considering the scientific and ethical values |
| LO14 | Using the assimilated horticulture molecular breeding knowledge in interdisciplinary studies, explaining, presenting and evaluating |
| LO15 | To be able to understand the concepts of ethics and biosafety in horticulture molecular breeding |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | - | To be able to develop and deepen knowledge acquired in horticultural field based on undergraduate level competencies | 0 |
| PLO02 | - | To be able to understand the interaction between horticultural field and related disciplines | 0 |
| PLO03 | - | To be able to combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative | 0 |
| PLO04 | - | Solving problems by using research methods and solving cause and effect relationships | 0 |
| PLO05 | - | To be able to conduct a study requiring expertise on horticultural plants independently | 0 |
| PLO06 | - | To develop an analytical approach for solving unpredictable complex problems encountered in horticultural applications, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained | 0 |
| PLO07 | - | To lead the horticultural plants in an environment that requires solving problems | 0 |
| PLO08 | - | Access to resources related to garden plants, benefiting from these resources and self-renewal | 0 |
| PLO09 | - | To be able to evaluate the knowledge and skills acquired in the field of horticulture with a critical approach and to direct the learning process | 0 |
| PLO10 | - | To be able to transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools | 0 |
| PLO11 | - | To be able to use advanced computer software and information and communication technologies as necessary in relation to horticultural plants | 0 |
| PLO12 | - | To be able to collect data with scientific methods for solving problems related to horticultural plants, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values. | 0 |
| PLO13 | - | To be able to use assimilated information in the field of horticulture in interdisciplinary studies, explain it, transfer it to others, examine the results from a critical perspective | 0 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | DNA, RNA theoretical expression, DNA isolation in plants | Reading the related lecture notes | |
| 2 | Introduction of molecular genetics laboratory, Laboratory Practice | Reading the related lecture notes | |
| 3 | Polymerase Chain Reactions, Electrophoresis Systems | Reading the related lecture notes | |
| 4 | Laboratory Practice: PCR optimization - preparation of PCR and determination of cycle conditions | Reading the related lecture notes | |
| 5 | Biochemical markers and their use in plant breeding, marker systems based on hybridization and their use in breeding (RFLP) | Reading the related lecture notes | |
| 6 | Laboratory Practice: Preparation of agarose and polyacrylamide gel | Reading the related lecture notes | |
| 7 | Molecular Markers (RAPD, ISSR, SRAP) | Reading the related lecture notes | |
| 8 | Mid-Term Exam | Preparation for the exam | |
| 9 | Laboratory Practice: RAPD, ISSR and SRAP | Reading the related lecture notes | |
| 10 | 38/5000 Molecular Markers (SSR, AFLP, SSCP) | Reading the related lecture notes | |
| 11 | Laboratory Practice: SSR, AFLP and SSCP | Reading the related lecture notes | |
| 12 | 36/5000 Genetic Mapping in Plants and MAS | Reading the related lecture notes | |
| 13 | Laboratory Practice: Genetic Maps | Reading the related lecture notes | |
| 14 | Methods for Determining Gene Expression in Plants -cDNA AFLP, Microarray, RNA seq, Real Time PCR | Reading the related lecture notes | |
| 15 | Laboratory Practice: Real Time PCR | Reading the related lecture notes | |
| 16 | Term Exams | Preparation for the exam | |
| 17 | Term Exams | Preparation for the exam |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 152 | ||
| Total Workload / 25 (h) | 6,08 | ||
| ECTS | 6 ECTS | ||