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Information
| Code | BTE0025 |
| Name | Principles of Gene Transfer in Plants |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | İngilizce |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
1 |
Course Goal / Objective
To teach the properties of plasmid vectors used in gene transfer and to teach what genes to be used in the analysis of transgenic plants and how to design primers.
Course Content
It includes the creation of plasmid vectors using recombinant DNA technologies, which are among the basic principles of gene transfer, the creation of conjugative and binary vector systems to be used in plant gene transfer, plant gene transfer methods and molecular testing of these genes. It covers the contents of vector constructs according to the methods used in gene transfer.
Course Precondition
No pre requirement
Resources
Principles of Gene manipulation (R.W. Old and S.B. Primrose, 1992), Technology transfer of plant biotechnology (Petter M. Gresshoff, NewYork, 1994), Transgenic plants research (Keith Lindsey 1997)
Notes
Course notes and articles
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns the general characteristics of plasmids originating from E. coli |
| LO02 | Learn the technique of gene cloning in a plasmid |
| LO03 | Learn how to transfer genes directly to plants and which vectors to use |
| LO04 | Learns the methods and details used in gene transfer to plants. |
| LO05 | Learns the properties of marker genes and how they should be inserted into vectors |
| LO06 | Learns how to insert promoter and terminator genes into genes used in gene transfer to plants and gene cassette |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. | |
| PLO02 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 1 |
| PLO04 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 4 |
| PLO05 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 3 |
| PLO06 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | 2 |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Gains the ability to develop and deepen their knowledge in the field of biotechnology at the level of expertise. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | E. coli bacteria containing plasmids | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 2 | Lamda DNA contructs | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 3 | Gen cloning techniques into plasmids | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 4 | Construction of gene cassette | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 5 | Direct gene transfer methods into plants | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 6 | Indirect gene transfer methods | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 7 | Marker genes | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 8 | Mid-Term Exam | Courses notes and related books | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Marker gene analysis | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 10 | Selection of transgenic plants and analysis | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 11 | Histochemical GUS assays | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 12 | Southern analysis of Transgenic plants | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 13 | Western Blotting of Transgenic plants | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 14 | Northern Blotting of Transgenic Plants | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Gösteri |
| 15 | Characteristic of transgenic plants produced in the world | Courses notes and related books | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Courses notes and related books | Ölçme Yöntemleri: Ödev |
| 17 | Term Exams | Courses notes and related books | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 5 | 70 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 15 | 15 |
| Total Workload (Hour) | 160 | ||
| Total Workload / 25 (h) | 6,40 | ||
| ECTS | 6 ECTS | ||