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Information
| Code | SY554 |
| Name | Molecular methods used in fish parasitology |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
The main purpose of this course is to teach the molecular techniques that will enable the most common parasites to be detected in economically valuable fish farming, faster than conventional parasite diagnosis methods, and to apply the right treatment as soon as possible.
Course Content
In this course, molecular techniques used for species identification of fish parasites are explained.
Course Precondition
none
Resources
Ed. Cunningham, C.O. 2002. Molecular Diagnosis of Salmonid Diseases. Kluwer Academic Publishers, Springer.
Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Recognizes parasitic diseases seen in economically valuable fish. |
| LO02 | Collects parasite samples from fish and isolate from fish tissues under the microscope. |
| LO03 | Recognizes the materials, devices and chemicals required for molecular analysis. |
| LO04 | Learns and comprehends the polymerase chain reaction technique. |
| LO05 | Learns DNA isolation protocols from different groups of parasites. |
| LO06 | Comprehends nucleic acid detection and labeling methods and the method of using enzymes. |
| LO07 | Learns the operation of RFLP, AFLP, RAPD methods. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Improves and deepens the institutional and practical knowledge in Aquaculture and Diseases | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehend the interdisciplinary interaction related to the field of expertise | 5 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Synthesizes and produces new theories with theoretical and practical knowledge specialized in Aquaculture and Diseases Departments. | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | It collects data about the subjects that it specializes in Aquaculture and Diseases Departments, evaluates the results, establishes cause and effect relationship and produces solutions. | 5 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Plans and conducts a study that requires expertise in Aquaculture and Diseases alone. It contributes to the solution of the problems that may occur as a specialist or as an individual or ancillary researcher and takes responsibility. | 3 |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | It reaches and uses resources related to Aquaculture and Diseases. | 4 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transmits the developments related to Aquaculture and Diseases in written, oral and visual form. | 1 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | He / she establishes written and verbal communication about the subject that he / she specializes in and he / she follows the meetings and seminars. | 4 |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | She/He develops strategy, policy and implementation plans in the fields of Aquaculture and Diseases, of which she/he is an expert, and evaluates the results obtained within the framework of total quality management processes. Uses the knowledge and experience gained in the fields of Aquaculture and Diseases, problem solving and/or application skills in interdisciplinary studies. | 4 |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Develops a positive attitude towards lifelong learning. | 2 |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Gains the competence to develop plans and design proposals sensitive to society, space and nature for different types of production. | 1 |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | She/he gains the ability to develop and deepen her knowledge in the fields of Aquaculture and Diseases, in which she/he is an expert. | 5 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Parasites in economically valuable fish - I | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 2 | Parasites in economically valuable fish - II | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 3 | Parasite examination and sampling in fish | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 4 | Parasite isolation in the microscope and fixation | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 5 | Molecular analysis methods for species identification | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım |
| 6 | Materials, devices and chemicals required for molecular analysis | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 7 | Polymerase chain reaction | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 8 | Mid-Term Exam | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | DNA isolation techniques from different parasite species | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 10 | RFLP technique | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 11 | AFLP tekniği | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 12 | RAPD tekniği | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 13 | DNA microarrays | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım |
| 14 | Potential problems and avoiding contamination | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım |
| 15 | Result evaluation | It will be prepared from the sources given. | Öğretim Yöntemleri: Anlatım, Gösteri |
| 16 | Term Exams | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||