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Information
| Code | HİS625 |
| Name | Molecular Biology Techniques |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. LEMAN SENCAR GÜNDOĞDU |
Course Goal / Objective
Getting information about molecular structure of the cell and learning main techniques that is frequently used in molecular biology.
Course Content
Molecular structures and functions of the cell, biomolecules, genom structure, function and structure of DNA and RNA, transcription, translation, DNA isolation, agarose gel electrophoresis, types and usage areas of PCR, blotting techniques.
Course Precondition
None
Resources
Michael H. Ross Histoloji Konu Lecture and Atlas
Notes
Lecture notes and slides
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows molecular structure of the cell. . |
| LO02 | Knows biomolecules notion. |
| LO03 | Getting information about structure of genom and nucleic acids |
| LO04 | Gettin information about DNA isolation and agarose gel electrophoresis |
| LO05 | Knows types and usage areas of polimerase chain reaction. |
| LO06 | Gettin information about ELISA method |
| LO07 | Explaining blotting techniques. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have knowledge about structure and function of tissue and organ systems. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Have knowledge about implantation biology and IVF techniques. | 5 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains knowledge of the normal development of tissue and organ systems, developmental disorders and mechanisms. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows advanced light and electron microscopic tissue preparation, histochemical, immunohistochemistry and cell culture techniques according to the study area. | 5 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Has knowledge about the basic properties of stem cells. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has advanced knowledge about technological tools, devices and tools used in histological studies | 4 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Knows the importance of ethical rules and ethical principles. | |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has knowledge about planning of experimental studies and statistical methods. | 3 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Prepares and examines the tissues using electron microscopic, immunohistochemistry and special staining techniques. | |
| PLO10 | Bilgi - Kuramsal, Olgusal | Usestechnological tools, devices and equipment according to the level. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Prepares experimental studies as a project. | 2 |
| PLO12 | Bilgi - Kuramsal, Olgusal | Presents the results of research in scientific meetings and publishes them in refereed journals. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Makes critical analysis, synthesis and evaluation related to the field. | |
| PLO14 | Bilgi - Kuramsal, Olgusal | Carries out studies individually or as a team member and leads the necessary environments. | |
| PLO15 | Bilgi - Kuramsal, Olgusal | Bilimsel bir çalışmayı bağımsız olarak planlayıp, gerçekleştirerek istatistiksel analizlerini yapar. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Uses the principles of professional development and lifelong learning in their studies. | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | Uses critical thinking, decision making and evidence-based medicine applications in their research. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | By communicating effectively, discusses the issues related to the field and defends their views. | |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Works with relevant disciplines in the process of selecting, applying and interpreting appropriate methods for studies and establish functional communication. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Molecular structures and functions of the cell | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 2 | Biomolecules | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 3 | Genom structure; Features and structures of nucleic acids | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 4 | DNA replication, DNA damage and repair mechanisms | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 5 | Initiation, elongation and termination of transcription | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 6 | Processing of mRNA, types of noncodding RNA | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 7 | DNA isolation and agorose gel electrophoresis | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev, Proje / Tasarım |
|
| 9 | Gene amplification with PCR, types and usage areas of PCR | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 10 | Isolation types of RNA and analysis methods | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 11 | Types of blotting; Western and Southern blot, micoarray techniques | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 12 | Structure and function of proteins | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 13 | Definition, importance and methods of epigenetics | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 14 | ELISA technique | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 15 | Seminar | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev, Proje / Tasarım |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev, Proje / Tasarım |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 7 | 7 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 109 | ||
| Total Workload / 25 (h) | 4,36 | ||
| ECTS | 4 ECTS | ||