TÜRKÇE
ENGLISH
Information
| Code | HİS514 |
| Name | Introduction to cell culture |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. LEMAN SENCAR GÜNDOĞDU |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is learning the cell culture techniques and applying them.
Course Content
Cell culture types, usage of Laminar Hood and Carbondioxide incubators, usage of Invert Microscope, obtaining and producing of the cells, mediums, serums and antibiotics used in cell culture, cell culture sterilization techniques.
Course Precondition
None
Resources
Michael H. Ross Histoloji Lecture and Atlas
Notes
Lecture notes and slides
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knowss the equipments required for a cell culture laboratory |
| LO02 | Learns the rules of the cell culture laboratory |
| LO03 | Learns the fundamental cell culture techniques |
| LO04 | Applies basic cell culture methods |
| LO05 | Solves the problems in the cell culture laboratory |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Tell the basic histological features of cells and tissues. | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Can establish connection betwwen the structure and function of the cells. | 3 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Have knowledge about the histological properties of organ systems. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Have knowledge about embryo development, development disorders and mechanisms. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Define light and electron microscopic tissue preparation and examination methods. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has knowledge about general principles of cell culture, histochemistry and immunohistochemistry. | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Prepare tissues for light and electron microscopic analysis. | 3 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Apply basic histochemical staining methods. | 4 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Can distinguish tissue and organ systems at light microscopic level. | |
| PLO10 | Bilgi - Kuramsal, Olgusal | He / she carries out his / her study as an individual or a team member. | |
| PLO11 | Bilgi - Kuramsal, Olgusal | Do the tasks given within the scope of research projects. | |
| PLO12 | Beceriler - Bilişsel, Uygulamalı | Develop a positive attitude and behavior related to lifelong learning. | 2 |
| PLO13 | Beceriler - Bilişsel, Uygulamalı | Identifies learning needs and resources, and guides them by using resources effectively. | |
| PLO14 | Beceriler - Bilişsel, Uygulamalı | Evaluate the knowledge and skills at the graduate level with a critical approach. | |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Use information and communication technologies at the level required by the field. | |
| PLO16 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Communicate effectively. | |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transfer their ideas and solutions by quantitative and qualitative datas by supporting the current developments and their studies orally and visiually | |
| PLO18 | Yetkinlikler - Öğrenme Yetkinliği | Knows the importance of ethical principles in conducting research studies, evaluating and sharing the results. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Cell culture and usage areas of it | Self-study, reading recommended references | |
| 2 | Historical events in development of the cell culture | Self-study, reading recommended references | |
| 3 | Types of cell cultures | Self-study, reading recommended references | |
| 4 | Primary culture of specific cell types | Self-study, reading recommended references | |
| 5 | Mediums, serum and antibiotics used in cell culture | Self-study, reading recommended references | |
| 6 | Culture dishes and flasks | Self-study, reading recommended references | |
| 7 | The usage of incubators | Self-study, reading recommended references | |
| 8 | Mid-Term Exam | Self-study, reading recommended references | |
| 9 | Contamination problems in cell culture laboratory | Self-study, reading recommended references | |
| 10 | Sterilization in cell culture laboratory | Self-study, reading recommended references | |
| 11 | Maintenance of cell lines and safety | Self-study, reading recommended references | |
| 12 | Cryopreservation of the cells for storage | Self-study, reading recommended references | |
| 13 | Thawing and reproducing of the cells | Self-study, reading recommended references | |
| 14 | Cell viability and counting of the cells | Self-study, reading recommended references | |
| 15 | Microscopic evaluation of cells | Self-study, reading recommended references | |
| 16 | Term Exams | Self-study, reading recommended references | |
| 17 | Term Exams | Self-study, reading recommended references |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 15 | 15 |
| Total Workload (Hour) | 118 | ||
| Total Workload / 25 (h) | 4,72 | ||
| ECTS | 5 ECTS | ||