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Information
| Code | BİO528 |
| Name | Molecular Genetics Analysis Methods |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-4 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. AYFER PAZARBAŞI |
Course Goal / Objective
Causing the student to gain knowledge, ability and manner of isolation of genomic material, analysis methods, interpret and application field.
Course Content
Genom analysis methods, Mechanism and optimization of Polymerase chain reaction (PCR), isolation of DNA, Agarose and polyacrilamid gel electrophoresis of PCR product, preparing the solutions and gels, staining the gels, QF-PCR method and analyse and interpret of QF-PCR results, Types of PCR and fields of application (Mutation screening, PCR-RFLP, Sequencing, Gene cloning), The method and applications of Real time PCR, Blotting Techniques.
Course Precondition
There is no precondition for this lesson.
Resources
Kasap H.(Ed.), M. Kasap, O. Demirhan, D. Alptekin, Ü. Lüleyap, A. Pazarbaşı, A.Irfan Güzel. Tıbbi Biyoloji ve Genetik, Nobel Kitabevi; ISBN: 978-605-397-059-0, 421-435, Adana (2016).
Notes
Related literatures
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | The student lists genome analysis methods. |
| LO02 | The student organizes the mechanism and optimization of polymerase chain reaction (PCR) |
| LO03 | Beeing able to apply DNA isolation , |
| LO04 | The student applies agarose and polyacrilamide gel electrophoresis of PCR product. |
| LO05 | The student prepares the solutions and gels, stains the gels, |
| LO06 | The student applies QF-PCR method. |
| LO07 | The student analyses and interprets the results of PCR and QF PCR |
| LO08 | The student sequences the types of PCR. |
| LO09 | The student sequences the fields of application (Mutation screening, PCR-RFLP, Sequencing, Gene cloning) |
| LO10 | Beeing able to define real-time PCR technique |
| LO11 | Beeing able to explain blotting techniques. |
| LO12 | Beeing able to define sequencing techniques. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To comprehend the structure and functions of cells and organelles which constitute the basic structure of life | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To recognize the functions of tissue and organ systems | |
| PLO03 | Bilgi - Kuramsal, Olgusal | To examine the structure of genetic material | 4 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To recognize genetic diseases and genes that cause these diseases | 1 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To learn the structure of genes | 1 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To define chromosome and gene mutations | 1 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To classify heredity forms (autosomal dominant, autosomal recessive, gender-dependent dominant and recessive inheritance models) | |
| PLO08 | Bilgi - Kuramsal, Olgusal | To use molecular and cytogenetic analysis | 1 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To be able to explain the numerical and structural anomalies of autosomal and sex chromosomes | |
| PLO10 | Bilgi - Kuramsal, Olgusal | To make DNA isolation from all kinds of tissues | 3 |
| PLO11 | Bilgi - Kuramsal, Olgusal | To prepare PCR | 3 |
| PLO12 | Bilgi - Kuramsal, Olgusal | To make and interpret agarose and polyacrylamide gel electrophoresis | 3 |
| PLO13 | Bilgi - Kuramsal, Olgusal | To measure the obtained DNA on the spectrophotometer | 2 |
| PLO14 | Bilgi - Kuramsal, Olgusal | To apply and interpret QF-PCR technique | 3 |
| PLO15 | Bilgi - Kuramsal, Olgusal | To make cell culture from blood, amnion, chorionic villus biopsy specimens and to obtain chromosome preparation | |
| PLO16 | Bilgi - Kuramsal, Olgusal | To perform karyotype analysis of chromosome preparations and to recognize and interpret all human chromosomes | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | To interpret a scientific article and to learn how to write a paper |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Molecular genetics diagnosis | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | DNA isolation | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | PCR, Mechanism and history, components of PCR | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | Designing of primers, Preparing and doing of PCR reaction | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | Agarose and polyacrilamide gel elecrophoresis of PCR product | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | Optimization of PCR | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 7 | Analysis of PCR results, troubleshoot | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Preparation of presentation | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 9 | Application fields (Mutation screening, RFLP, Allele specific oligonucleotides: ASO) | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Mutation screening | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 11 | Gene cloning | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 12 | Types of PCR (Multiplex, QF-PCR, Real -time, nested) | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | DNA sequencing | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Blotting techniques. | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | Next Generation Sequencing | Literature rewiev and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Preparation of multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation of multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 3 | 15 | 45 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 2 | 2 |
| Total Workload (Hour) | 147 | ||
| Total Workload / 25 (h) | 5,88 | ||
| ECTS | 6 ECTS | ||