ABL581 Experimental Biochemistry

5 ECTS - 3-0 Duration (T+A)- . Semester- 3 National Credit

Information

Code ABL581
Name Experimental Biochemistry
Semester . Semester
Duration (T+A) 3-0 (T-A) (17 Week)
ECTS 5 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator


Course Goal

She/He gains an effective understanding of special analysis techniques used in biochemistry, and gain research competence in the field of forensic biochemistry.

Course Content

In this course, basic methods used in biochemistry laboratory are given. The topics that are based on the computer and internet use in biochemistry researches, general laboratory procedures, centrifugation techniques, purification and analysis of biomolecules by chromatographic methods, characterization of proteins and nucleic acids by electrophoresis, spectroscopic analysis of biomolecules, interaction of biomolecules, structure and analysis of nucleic acids, recombinant DNA, molecular cloning and enzymology, purification and characterization of proteins and discussion of current scientific publications are explained.

Course Precondition

There is no prerequisite.

Resources

Switzer, R. L; Garrity, L.F. Experimental biochemistry (theory and exercises in fundamental ), 3. Ed. New York, NY Freeman, 1999.; Boyer, Rodney F. Biochemistry laboratory : modern theory and techniques. 2nd ed. Pearson Education, Inc. 2012

Notes

www.sciencedirect.com; www.scopus.com; www.webofknowledge.com


Course Learning Outcomes

Order Course Learning Outcomes
LO01 She/ He explains what to pay attention to when working with biological materials.
LO02 She/He recognizes the devices in the laboratory.
LO03 She/He calculates the results of the analysis by considering scientific notation.
LO04 She/He uses her decision-making skills.
LO05 She/He gains research skills.
LO06 She/He recognizes the material and device used in the analysis method.
LO07 She/HeIt counts spectroscopic basis analysis analysis.
LO08 She/He explains the basis of spectroscopic analysis.
LO09 She/He counts the factors affecting the behavior of biological samples in the electrophoretic field.
LO10 She/He explains the basics of electrophoretic techniques.
LO11 She/He explains the basic working principles of instrumental analysis devices used in the analysis of samples.


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Knows current concepts in the field of Forensic Sciences. 2
PLO02 Bilgi - Kuramsal, Olgusal Comprehends the relations between the areas of Forensic Sciences. 2
PLO03 Bilgi - Kuramsal, Olgusal Comprehends the importance of ethical principles and ethical committees for the individual and society.
PLO04 Bilgi - Kuramsal, Olgusal Knows the concept of expertise and forensic procedural approach.
PLO05 Bilgi - Kuramsal, Olgusal Recognize the statistical methods that are frequently used in studies in the field of Forensic Sciences.
PLO06 Bilgi - Kuramsal, Olgusal Knows crime scene investigation and evidence collection procedures
PLO07 Beceriler - Bilişsel, Uygulamalı Interprets the knowledge gained in the field by integrating it with the knowledge from different disciplines. 3
PLO08 Beceriler - Bilişsel, Uygulamalı Uses statistical software that is frequently used in the field of Forensic Sciences.
PLO09 Beceriler - Bilişsel, Uygulamalı Uses the knowledge and research methods obtained in the field in solving forensic cases. 3
PLO10 Beceriler - Bilişsel, Uygulamalı Takes responsibility by participating in teamwork
PLO11 Beceriler - Bilişsel, Uygulamalı Develops solutions for solving complex problems that may be encountered in the field of Forensic Sciences. 3
PLO12 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Can lead in certain challenging Forensic circumstances include interdisciplinary ones. 3
PLO13 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Follow the scientific meetings and researches in forensic area. 3
PLO14 Yetkinlikler - Alana Özgü Yetkinlik Discusses current developments and studies with different groups in written, oral and visual formats. 3
PLO15 Yetkinlikler - Alana Özgü Yetkinlik Uses current developments, information and practices in Forensic Sciences for the benefit of society. 2


Week Plan

Week Topic Preparation Methods
1 Safety in the Laboratory. Keeping Records and Communicating Experimental Results.Using Biochemical Reagents and Solutions. Quantitative Transfer of Liquids. Statistical Analysis of Experimental Data Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
2 Using Computers in Biochemistry. Web Sites Useful in Biochemistry Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
3 pH, Buffers, Electrodes, and Biosensors. Measurement of Protein Solutions. Measurement of Nucleic Acid Solutions. Techniques for Sample Preparation. Radioisotopes in Biochemistry Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
4 The centrifugation techniques in biochemistry Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
5 Chromatography and mass spectrometry Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
6 HPLC method Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
7 Ion exchange chromatography Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap, Tartışma
8 Midterm Studying lecture notes. Ölçme Yöntemleri:
Ödev
9 Characterization of proteins by electrophoresis Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
10 Nucleic acid purification and characterization by electrophoretic technique Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
11 Spectroscopic analysis of biomolecules Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
12 Biomolecular interactions Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
13 Basic studies of molecular biology Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
14 Diagnostic techniques in DNA Reading the relevant part of the textbook. Öğretim Yöntemleri:
Anlatım, Soru-Cevap
15 Current literature discussion on studies in biochemistry in Forensic Sciences Examination of current scientific studies from scientific databases. Öğretim Yöntemleri:
Tartışma
16 Biochemical calculations Sample question solution from the relevant textbooks of the topics covered in the course Öğretim Yöntemleri:
Soru-Cevap
17 Final Re-studying the topics covered in the course from the relevant textbooks and lecture notes. Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 3 42
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 18 18
Total Workload (Hour) 114
Total Workload / 25 (h) 4,56
ECTS 5 ECTS