BT0096 Polymerase Chain Reaction (PCR), display of the Optimization Results

6 ECTS - 3-0 Duration (T+A)- . Semester- 3 National Credit
Information About Course

Information
Goal, Content & Resources
Course Learning Outcomes
Relation with Program Learning Outcome
Week Plan
Student Workload - ECTS

Information

Code BT0096
Name Polymerase Chain Reaction (PCR), display of the Optimization Results
Semester . Semester
Duration (T+A) 3-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator


Course Goal

The aim of the course is to enable the graduates to have knowledge about Polymerase Chain Reaction, to be able to apply the test and to solve the problems they may encounter during the applications.

Course Content

The components of this course consist of Polymerase Chain Reaction, it's standardisation and the evaluation of the test results.

Course Precondition

NONE

Resources

Lecture notes and presentations will be used on for the courses

Notes

Freeman S, Herron JC, Evrimsel Analiz, Palme Yayıncılık, Ankara, 2009. Lemey P, Salemi M, Vandamme AM, The Phylogenetic Handbook, A Practical Approach to Phylogenetic Analysis and Hypothesis Testing, Cambridge University Press, 2009. Pevsner J, Bioinformatics and Functional Genomics, Wiley-Blackwell, 2009. Xıong J, Essential Bioinformatics, Cambridge University Press, 2006.


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Student can perform electrophoresis and imaging processing of PCR products
LO02 Students will solve the problems while they are using PCR and optimise the test
LO03 Students will have a knowledge about PCR and PCR based molecular techniques
LO04 Students will learn DNA ekstraction from the tissues
LO05 Students will learn the molecular structure of the cell


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods. 3
PLO02 Beceriler - Bilişsel, Uygulamalı To identifiy the interdisciplinary interaction of the field and to solve use of the methods. 3
PLO03 Beceriler - Bilişsel, Uygulamalı Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information 3
PLO04 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. 3
PLO05 Yetkinlikler - Öğrenme Yetkinliği To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. 3
PLO06 Yetkinlikler - İletişim ve Sosyal Yetkinlik To solve the problems encountered in the field and cominicate by using research methods. 2
PLO07 Yetkinlikler - Alana Özgü Yetkinlik To gain the ability to develop and deepen the knowledge in the field of biotechnology 2
PLO08 Yetkinlikler - Alana Özgü Yetkinlik Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field.
PLO09 Yetkinlikler - Öğrenme Yetkinliği Knows the ethical rules to be considered while obtaining biotechnological products.
PLO10 Yetkinlikler - İletişim ve Sosyal Yetkinlik He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field.


Week Plan

Week Topic Preparation Methods
1 Structure of the cell Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
2 The structure and replication of of DNA Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
3 Transcription and translation Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
4 Gen, Genom and Regulation of DNA Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
5 Mutations (Genom, Gen and DNA mutations) Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
6 DNA extraction methods DNA extraction methods Öğretim Yöntemleri:
Anlatım, Soru-Cevap
7 Introduction to Polymerase Chain Reaction (PCR) Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
8 Mid-Term Exam Mid-Term Exam Ölçme Yöntemleri:
Sözlü Sınav, Ödev
9 Modifications of PCR (Semi-Nested PCR, Nested PCR, Multiplex-PCR, RAPDPCR, Real Time-PCR) Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
10 Optimisation in PCR ( enzyme, buffer, magnesium chloride, potassium chloride, dNTP mix, primers) Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
11 Optimisation in PCR (Optimisation of PCR cycles) Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
12 Preparation of gels (agarose, PAGE) that used in the electrophoresis of the amplicons Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
13 Electrophoresis, staining of PCR gels and screanin of the results Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
14 Optimisation in the electrophoresis, jel preparation and screening Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
15 Problems in PCR and solution offers Lecture notes,slide projection Öğretim Yöntemleri:
Anlatım, Soru-Cevap
16 Term Exams Term Exams Ölçme Yöntemleri:
Sözlü Sınav, Ödev
17 Term Exams Term Exams Ölçme Yöntemleri:
Yazılı Sınav, Sözlü Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 3 42
Out of Class Study (Preliminary Work, Practice) 14 5 70
Assesment Related Works
Homeworks, Projects, Others 0 0 0
Mid-term Exams (Written, Oral, etc.) 1 15 15
Final Exam 1 30 30
Total Workload (Hour) 157
Total Workload / 25 (h) 6,28
ECTS 6 ECTS