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Information
| Code | DM0010 |
| Name | Basic Molecular Biology Techniques for Tissue Engineering II |
| Term | 2023-2024 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
To learn the basic applications of molecular biology techniques in the laboratory.
Course Content
Investigation of Application Areas of Basic Molecular Biology Techniques
Course Precondition
None
Resources
Basic and Advanced Molecular Biology Methods: Genomic and Proteomic Analyses, Nobel Medical Bookstore
Notes
Genomic and Proteomic Analyses, Nobel Medical Bookstore
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Has knowledge of basic molecular biology techniques |
| LO02 | Knows in which researches the basic methods in molecular biology are used |
| LO03 | Has knowledge about the laboratory conditions under which molecular biology applications can be performed |
| LO04 | Comprehends the scientific importance of molecular biological methods |
| LO05 | Knows current molecular biological approaches and has knowledge about literature review |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Yetkinlikler - Alana Özgü Yetkinlik | Having advanced knowledge of the concepts, theories and principles of the Tissue Engineering Department | 3 |
| PLO02 | Yetkinlikler - Alana Özgü Yetkinlik | Being equipped with advanced mathematics, science and engineering knowledge | |
| PLO03 | Yetkinlikler - Alana Özgü Yetkinlik | To be able to design and perform advanced experiments in the Tissue Engineering Department; To be able to analyze and interpret the results and the data obtained. | 3 |
| PLO04 | Yetkinlikler - Alana Özgü Yetkinlik | Be equipped with a variety of advanced engineering techniques and skills | |
| PLO05 | Yetkinlikler - Alana Özgü Yetkinlik | Ability to lead the definition, design, development and deployment of a product or production method | 2 |
| PLO06 | Yetkinlikler - Alana Özgü Yetkinlik | Being aware of the necessity of lifelong learning and being able to constantly renew oneself | |
| PLO07 | Yetkinlikler - Alana Özgü Yetkinlik | Ability to use English effectively both verbally and in writing on technical or non-technical subjects | 3 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Being prone to teamwork, being able to communicate well with the environment; Ability to work efficiently as a member of a multi-faceted and interdisciplinary team | 5 |
| PLO09 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Being curious and innovative, aware of its technical and ethical responsibilities | 4 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Gains high-level skills in using research methods in studies related to the field of Tissue Engineering. | 5 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Discovery of DNA and its use in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Discovery RNA and its use in molecular biology | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 3 | Obtaining DNA and RNA from the cell | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Obtaining protein from the cell | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 5 | Extraction of DNA and RNA from tissue | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 6 | Protein extraction from tissue | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Review of molecular biological methods in the literatureReview of molecular biological methods in the literature | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 9 | Quantification of DNA and RNA | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | PCR types and applications | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Agarose gel electrophoresis | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Eliza varieties and application method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | DNA sequencing methods and next generation sequencing | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 14 | Western blot method | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Researching studies involving current molecular biological techniques | Literature browsing and reading | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 16 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
|
| 17 | Term Exams | Ölçme Yöntemleri: Performans Değerlendirmesi |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 6 | 12 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||