TÜRKÇE
ENGLISH
Information
| Code | BBP352 |
| Name | Molecular Breeding Methods in Horticulture |
| Term | 2024-2025 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. SALİH KAFKAS |
| Course Instructor |
1 2 Prof. Dr. SALİH KAFKAS (A Group) (Asst. Ins.) |
Course Goal / Objective
The use of molecular techniques in plant breeding and a detailed examination of these techniques
Course Content
This lesson includes topics: Analysis of DNA, RNA structures, PCR mechanism, genetic markers: morphological and biochemical markers, molecular marker technologies and genetic mapping from molecular methods, their use in early selection and breeding with the help of markers, methods used in determining plant gene expressions and next generation sequencing methods and their use in plant breeding
Course Precondition
There are no prerequisites for the course
Resources
Lecture notes, books, research articles, periodicals on the subject
Notes
Internet resources obtained from scientific search engines
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Compare DNA and RNA in terms of similarities and differences. |
| LO02 | Defines molecular marker techniques. |
| LO03 | Defines and tests Polymerase Chain Reaction. |
| LO04 | Explains the methods of electrophoresis |
| LO05 | Memorises molecular breeding methods based on markers in horticultural plant breeding. |
| LO06 | Expresses an opinion on the use of genetic mapping in breeding. |
| LO07 | Refers to the idea of knowledge about selection by markers (MAS). |
| LO08 | Interpret the issues related to the use of gene expression methods in breeding. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Adequate knowledge on subjects specific to the discipline of Mathematics, Science and Agricultural Engineering (Horticulture), ability to use theoretical and applied knowledge in these fields in complex engineering problems | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | The ability to identify and solve problems related to the cultivation, breeding and product preservation of fruit, vegetables, vineyards and ornamental plants in horticulture, the ability to choose and apply appropriate analysis and modeling methods for this purpose. | 3 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | The ability to design in a way that meets the necessary conditions for the cultivation of fruit, vegetables, vineyards and ornamental plants in the open and greenhouse in horticulture and the ability to apply modern design methods for this purpose. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Ability to select and use modern tools necessary for the analysis and solution of complex problems encountered in horticulture practices, ability to use information technologies effectively | |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Ability to design and conduct experiments, collect data, analyze and interpret results for the study of complex problems or discipline-specific research issues in the field of Horticulture | |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Breeding of Horticultural Plants, developing new varieties, making selection, protecting genetic resources, producing propagation materials (seeds, seedlings, saplings) of developed varieties, ability to work in individual and multi-disciplinary teams | 3 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Ability to write effective reports in the field of Horticulture, to understand written reports, to prepare design and production reports, to make effective presentations, to take and give clear and understandable instructions | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Awareness of the necessity of lifelong learning, the ability to access information in the field of Horticulture, to follow the developments in science and technology and to constantly renew oneself | 1 |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Behaving in accordance with ethical principles, professional and ethical responsibility in the field of Horticulture, and knowledge of standards used in engineering practices | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Information about applications in business life, such as project management, risk management and change management in the field of Horticulture, awareness of entrepreneurship, innovation, information about sustainable development | |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Knowledge of the effects of horticultural practices on health, environment and safety in universal and social dimensions and the problems of the age reflected in the field of Horticulture, awareness of the legal consequences of horticultural solutions |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | DNA and RNA structure | Reading of the papers about the lecture. | Öğretim Yöntemleri: Anlatım |
| 2 | DNA and RNA isolation and methods used for determination the amount of DNA and RNA | Reading of the papers about the lecture. | Öğretim Yöntemleri: Anlatım |
| 3 | The genetic markers and the history | Reading of the papers about the lecture. | Öğretim Yöntemleri: Anlatım |
| 4 | PCR technologies and the use area | Reading of the papers about the lecture. | Öğretim Yöntemleri: Anlatım |
| 5 | Electrophoresis systems | Reading of the papers about the lecture | Öğretim Yöntemleri: Anlatım |
| 6 | Molecular marker technologies and the use in the plant breeding | Reading of the papers about the lecture | Öğretim Yöntemleri: Anlatım |
| 7 | PCR-based molecular markers | Reading of the papers about the lecture | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Preparation to mid-term exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | The creation of genetic maps | Reading of the papers about the lecture | Öğretim Yöntemleri: Anlatım |
| 10 | The use of genetic maps in plants breeding | Reading of the papers about the lecture | Öğretim Yöntemleri: Anlatım |
| 11 | Marker assistant selection for plant breeding | Reading of the papers about the lecture | Öğretim Yöntemleri: Anlatım |
| 12 | Molecular methods used in determination gene expression in plant and the use in plant breeding | Reading of the papers about the lecture | Öğretim Yöntemleri: Anlatım |
| 13 | Next generation sequencing technologies | Reading of the papers about the lecture. | Öğretim Yöntemleri: Anlatım |
| 14 | The use of next generation sequencing technology in plant breeding | Reading of the papers about the lecture. | Öğretim Yöntemleri: Anlatım |
| 15 | General review | Reading of the papers about the lecture. | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Preparation to final exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation to final exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 78 | ||
| Total Workload / 25 (h) | 3,12 | ||
| ECTS | 3 ECTS | ||