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Information
| Code | BL310 |
| Name | Molecular Biology |
| Term | 2024-2025 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Label | C Compulsory |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. BURHAN ARIKAN |
| Course Instructor |
1 2 |
Course Goal / Objective
The aim of this course is to teach the student topics such as the structure of macromolecules, their analysis, repair systems, proteomics, genomics and antisense oligonucleotide method and molecular typing.
Course Content
In this course, DNA structure and replication, DNA replication enzymes, DNA sequence analysis, Restriction endonuclease enzymes, Protein isolation techniques and purification, DNA and Protein Analysis Techniques Agarose gel electrophoresis, PAGE, isoelectric focusing, two-dimensional electrophoresis, gradient PAGE, DNA repair mechanisms SOS , Excision, Photoreactivation, Post replicative repair, Proteomics, Genomics, Gene therapy and used methods, Antisense oligonucleotide technology, Recombinant vaccines, Recombinant DNA and Recombinant Virus vaccines are covered.
Course Precondition
none
Resources
Molecular biology, Gene cloning and DNA Analysis
Notes
Techniques used in molecular biology
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Makes DNA replication and replication types |
| LO02 | Molecular content of DNA replications |
| LO03 | makes Protein isolation techniques and prufication |
| LO04 | Molecular identification techniques (DNA fingerprint, RFLP, RAPD) |
| LO05 | Proteomic, Genomic and gene theraphy |
| LO06 | DNA repair mechanisms (SOS, Exiition, Photoreactivation, Post replicatice repair) |
| LO07 | Antisence oligonucleotide techniques |
| LO08 | Makes PAGE, SDS-PAGE, two dimensional PAGE, İsoelectric focusing, Immunelectrophoresis |
| LO09 | grasp Antisence oligonucleotide techniques |
| LO10 | Recombinant DNA vaccines |
| LO11 | Recombinant virus vaccines |
| LO12 | grasp Molecular identification techniques (DNA fingerprint, RFLP, RAPD) |
| LO13 | Learns Proteomics, Genomics and gene therapy |
| LO14 | Makes DNA and protein anaysis techniques (Starch, cellulosa asetate, agarose gel electrophoresis |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Explain the basic concepts and principles in biology | |
| PLO02 | Bilgi - Kuramsal, Olgusal | The knowledge gained in the field of biology combine with information from other sciences. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Identifies problems in the field of biology and apply methods for their solution | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Describes the method of producing scientific knowledge in the field of biology | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | They will receive information on the biology area, in order to classify it uses and purposes. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | They use advanced theoretical and practical knowledge that obtain in biology field. | 3 |
| PLO07 | Bilgi - Kuramsal, Olgusal | They are Field-related issues can inform the relevant persons and institutions, They share ideas and problems relating to suggestions as the written, oral and Visual to expert or transfer to non-experts. | |
| PLO08 | Bilgi - Kuramsal, Olgusal | They can take responsibility as individuals and team members to solve unpredictable problems encountered in applications related to the field. | |
| PLO09 | Bilgi - Kuramsal, Olgusal | they can take an active role as a member of the execution team the planning of projects related to the field. | 2 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | They know that life-long learning process and act accordingly. | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They will increase the knowledge and experience outside major issues with specific elective courses in different fields of biology. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They speak English enough to communicate with colleagues to understand the basic concepts of biology. | 2 |
| PLO13 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They both with colleagues, as well as areas with non-person establishes ethical uses of information and communication technology. In addition to professional development, in line with the interests and abilities of scientific, social, constantly improves itself by identifying learning needs in cultural and artistic fields. | |
| PLO14 | Yetkinlikler - Öğrenme Yetkinliği | They assesses developments in biology versatile using scientific methods and techniques | |
| PLO15 | Yetkinlikler - Öğrenme Yetkinliği | They know the importance of personal development and enforces the need. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | DNA replication and replication types | general information | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | Molecular content of DNA replications | Study in literature | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Beyin Fırtınası |
| 3 | DNA repcilation enzymes | Research on topics | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 4 | DNA sequencing | preparation of the section | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Gösteri, Beyin Fırtınası |
| 5 | Resctriction endonuclease enzymes | Preparation of related topics | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 6 | Protein isolation techniques and prufication | Study in technical | Öğretim Yöntemleri: Anlatım, Grup Çalışması, Deney / Laboratuvar, Bireysel Çalışma |
| 7 | PAGE, SDS-PAGE, two dimensional PAGE, İsoelectric focusing, Immunelectrophoresis | Practical experiment | Öğretim Yöntemleri: Anlatım, Tartışma, Deney / Laboratuvar, Bireysel Çalışma |
| 8 | Mid-Term Exam | preparation for related matters | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
| 9 | DNA repair mechanisms (SOS, Exiition, Photoreactivation, Post replicatice repair) | Study in related books | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 10 | Antisence oligonucleotide techniques | Reading of manuscript | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Beyin Fırtınası |
| 11 | Recombinant DNA vaccines | Study of books | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 12 | Recombinant virus vaccines | Researc of area | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 13 | Molecular identification techniques (DNA fingerprint, RFLP, RAPD) | Reading of topics | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Proteomic, Genomic and gene theraphy | Study in related topics | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası |
| 15 | DNA and protein anaysis techniques (Starch, cellulosa asetate, agarose gel electrophoresis) | Researching the relevant section | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Alıştırma ve Uygulama, Deney / Laboratuvar |
| 16 | Term Exams | Study in literature | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
| 17 | Term Exams | Study of all topics | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 6 | 6 |
| Final Exam | 1 | 13 | 13 |
| Total Workload (Hour) | 75 | ||
| Total Workload / 25 (h) | 3,00 | ||
| ECTS | 3 ECTS | ||