BY707 PAGE Tecniques

6 ECTS - 2-0 Duration (T+A)- . Semester- 2 National Credit

Information

Code BY707
Name PAGE Tecniques
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 2-0 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 2 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. HATİCE GÜVENMEZ


Course Goal / Objective

To teach protein electrophoresis techniques used in molecular biology applications

Course Content

Isolation of proteins from living tissues, solutions for isolation, electrophoresis techniques and applications

Course Precondition

None

Resources

Protein Methods

Notes

Current Literatures


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Comprehend the importance of Page
LO02 Understands the structure and formation of polyacrylamide gels
LO03 Comprehend the usage areas and purposes of polyacrylamide gel systems
LO04 comprehend the application of PAGE techniquee


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Based on the undergraduate level qualifications, develops and deepens the knowledge in the same or different field at the level of expertise and analyzes and interprets them using statistical methods.
PLO02 Bilgi - Kuramsal, Olgusal Identifies the interdisciplinary interaction that the field is related to. 4
PLO03 Bilgi - Kuramsal, Olgusal Interprets the knowledge gained in the field by integrating the information from different disciplines and creates new information. 3
PLO04 Bilgi - Kuramsal, Olgusal Carries out a study that requires expertise related to the field independently. 4
PLO05 Beceriler - Bilişsel, Uygulamalı Evaluates the knowledge and skills acquired at the level of expertise in the field with a critical approach and directs its learning. 5
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği Supports the current developments in the field and their studies with quantitative and qualitative data and transmits them both in the field and outside the field systematically in written, oral and visual form. 4
PLO07 Yetkinlikler - Öğrenme Yetkinliği It supervises the social, scientific, cultural and ethical values in the collection, interpretation, application and announcement stages of the data related to the field and teaches these values.
PLO08 Bilgi - Kuramsal, Olgusal The information they absorbed in the field; use problem solving and / or practical skills in interdisciplinary studies. 3
PLO09 Belirsiz It contributes to the solution of social, scientific, cultural and ethical problems encountered in the field and supports the development of these values.
PLO10 Belirsiz Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making.


Week Plan

Week Topic Preparation Methods
1 The buffers for proteins isolation reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Alıştırma ve Uygulama
2 Salts, Metal ions, Inhibitors and chelators reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Gösterip Yaptırma
3 proteinase inhibitors reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Gösterip Yaptırma
4 PAGE structure and formation reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım, Deney / Laboratuvar
5 Polymerization and catalizors reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Soru-Cevap, Gösterip Yaptırma
6 Physicochemical properties of gel forming components reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım
7 Preparation of separating and stacking gels reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Deney / Laboratuvar
8 Mid-Term Exam An overview Ölçme Yöntemleri:
Sözlü Sınav, Performans Değerlendirmesi
9 Preparation of the homogen, gradient, denature and native PAGE and running the protein samples reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Alıştırma ve Uygulama
10 Causes of errors in PAGE electrophoresis applications reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Anlatım
11 Detection methods of protein bands reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Alıştırma ve Uygulama
12 Cellulose asetate electrophoresis reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Alıştırma ve Uygulama
13 Isoelectric focusing reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Alıştırma ve Uygulama
14 Detection of enzyme activity in PAGE reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Alıştırma ve Uygulama
15 Drying the acriylamide gels and hiding. reading current lecture notes, related papers,and searching internet Öğretim Yöntemleri:
Alıştırma ve Uygulama
16 Term Exams An overview Ölçme Yöntemleri:
Sözlü Sınav, Performans Değerlendirmesi
17 Term Exams oral examination Ölçme Yöntemleri:
Sözlü Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 2 28
Out of Class Study (Preliminary Work, Practice) 14 6 84
Assesment Related Works
Homeworks, Projects, Others 4 6 24
Mid-term Exams (Written, Oral, etc.) 1 6 6
Final Exam 1 12 12
Total Workload (Hour) 154
Total Workload / 25 (h) 6,16
ECTS 6 ECTS

Update Time: 07.05.2024 02:59