TÜRKÇE
ENGLISH
Information
| Code | KM334 |
| Name | Biochemistry 2 Lab. |
| Term | 2024-2025 Academic Year |
| Semester | 6. Semester |
| Duration (T+A) | 0-4 (T-A) (17 Week) |
| ECTS | 2 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. RAMAZAN BİLGİN |
| Course Instructor |
1 2 Prof. Dr. RAMAZAN BİLGİN (A Group) (Asst. Ins.) |
Course Goal / Objective
The aim of this course is to provide students with the application and evaluation of basic biochemical analysis methods and the theoretical knowledge of experimental results.
Course Content
The aim of this course is to introduce the application and evaluation of basic biochemical analysis methods and experimental results with theoretical knowledge.
Course Precondition
Yok
Resources
Labaoratory text book
Notes
Labaoratory text book
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Understands biochemical analysis methods. |
| LO02 | Evaluates experimental results. |
| LO03 | Understands DNA isolation |
| LO04 | Understands the determination of molecular weight of proteins by SDS-PAGE electrophoresis. |
| LO05 | Understands Lowry protein method. |
| LO06 | Understands determination of lipase activity |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Relates his/her knowledge in the field of chemistry to life and industry by relying on his/her knowledge | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Evaluates the developments in the world by following and perceiving the orientations in the industry. | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains awareness of lifelong learning and renews his/her knowledge. | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Access to information and associate their knowledge with other fields of science. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Understands the need to combine information in the application of information and perceives the total quality awareness. | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | S/he has knowledge in the use of resources, in the creation of technology depending on the country's resources. | 3 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | S/he conveys what s/he knows to people close or distant to the subject by concretising. | 3 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Gains the ability to access new information and technology. | 3 |
| PLO09 | Bilgi - Kuramsal, Olgusal | Learns to think creatively and produce solutions to problems in the field. | |
| PLO10 | Yetkinlikler - Öğrenme Yetkinliği | To be able to associate information by having the ability of reasoning and transfer what they know to practice. | 4 |
| PLO11 | Beceriler - Bilişsel, Uygulamalı | Sets up laboratory equipment and makes decisions alone. | 4 |
| PLO12 | Bilgi - Kuramsal, Olgusal | To be able to follow the current technology in the field of chemistry and gain added value. | 3 |
| PLO13 | Bilgi - Kuramsal, Olgusal | Follows the developments, gains the ability to follow the directions in the field. | 3 |
| PLO14 | Bilgi - Kuramsal, Olgusal | Knowing information sharing and participating in teamwork and directing the team. | 2 |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | Gains universal thinking and critical perspective. | |
| PLO16 | Bilgi - Kuramsal, Olgusal | Starts to have a say in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Determination of vitamin C in fruit juice | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 2 | Determination of urea in serum | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 3 | Isolation of DNA | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 4 | Determination of Hemoglobin in whole blood | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 5 | Polarimeter experiment | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 6 | Determination of molecular weight of proteins with SDS-PAGE electrophoresis | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 7 | Determination of molecular weight of proteins with SDS-PAGE electrophoresis I | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Lowry protein method | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 10 | Determination of lipase activity by titration | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 11 | Immobilization of lipase onto Celite | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 12 | Immobilization of lipase onto Celite I | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 13 | Immobilization of lipase onto Celite II | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 14 | Make-up experiments | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 15 | Make-up experiments I | Biochemistry books, lecture notes | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 16 | Term Exams | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Study related lecture notes | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 1 | 14 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 4 | 4 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 58 | ||
| Total Workload / 25 (h) | 2,32 | ||
| ECTS | 2 ECTS | ||