TÜRKÇE
ENGLISH
Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (MASTER) (WITH THESIS) (INTERDISCIPLINARY) | |
| Code | BT077 |
| Name | Immunohistochemical and Immunocytochemical Techniques in Animal Tissues |
| Term | 2024-2025 Academic Year |
| Term | Fall |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MEHMET HALIGÜR |
| Course Instructor |
Prof. Dr. MEHMET HALIGÜR
(Güz)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
This course aims to identify proteins, antigens and antibodies found in animal tissue cells using immunohistochemical or immunocytochemical methods and to describe these reactions.
Course Content
This course includes immunohistochemical or immunocytochemical determination of antigen-antibody complexes, infectious agents and tumor markers in animal tissues and evaluation of the results obtained from these methods.
Course Precondition
None
Resources
Instructor course material, Lin F, Prichard J. 2015. Handbook of Practical Immunohistochemistry, Springer; Dabbs D. 2021. Diagnostic Immunohistochemistry, 6th Edition, Elsevier. Renshaw S. 2017. Immunohistochemistry and Immunocytochemistry: Essential Methods, 2nd Edition, Wiley-Blackwell Del Walle L. 2022. Immunohistochemistry and Immunofluorescence: Methods and Protocols, springer
Notes
Lecture notes and current literature
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Applies immunohistochemical method to determine protein, antibody and antigen in animal tissues. |
| LO02 | List the application stages of immunohistochemical and immunocytochemical methods. |
| LO03 | Distinguish which method to use in the detection of infectious diseases and neoplastic cells in animal tissues. |
| LO04 | It detects receptors on cells in tissues using immunohistochemical or immunocytochemical methods. |
| LO05 | Explain the characteristics of antibodies and antigens. |
| LO06 | Describe the types of immunohistochemical and immunocytochemical methods. |
| LO07 | Analyze the results of immunohistochemical and immunocytochemical methods. |
| LO08 | Prepares samples for use in immunohistochemical and immunocytochemical methods |
| LO09 | It outlines the molecular changes caused by disease agents in tissues and cells. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on undergraduate level qualifications, they develop their knowledge at the level of expertise in the same or a different field. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | 5 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 3 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 2 |
| PLO06 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | 2 |
| PLO07 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | 3 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Definition of immunohistochemistry and immunocytochemistry, purpose and purposes of use | No pre-preparation | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Definition and properties of antigen and antibody | Reading lecture notes, Reading book, Viewing visual data | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Gösteri |
| 3 | Classification and general characteristics of infectious agents | Reading lecture notes, Reading book | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Beyin Fırtınası, Alıştırma ve Uygulama |
| 4 | Sample processing methods for the application of immunohistochemistry and immunocytochemistry | Preparing for a lab, Reading book | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösteri, Deney / Laboratuvar |
| 5 | Types of immunohistochemistry and immunocytochemistry, PAP and APPAP methods, how to apply | Reading lecture notes, Preparing for a lab | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Alıştırma ve Uygulama, Gösterip Yaptırma, Deney / Laboratuvar |
| 6 | Immunohistochemical determination of infectious agents and methods to be used | Reading lecture notes, Reviewing visual materialsReading book | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Gösterip Yaptırma, Benzetim |
| 7 | Points to be considered when determining the method to be preferred (immunohistochemical or immunocytochemical methods) in determining the receptors found in cells. | Reading lecture notes, Reviewing current literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Problem Çözme, Alıştırma ve Uygulama |
| 8 | Mid-Term Exam | Preparing for an exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Performans Değerlendirmesi, Ödev |
| 9 | General characteristics of tumors and types of metastasis | Reading lecture notes, Reviewing visual materials | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | In immunocytochemical and immunocytochemical methods, general biomarkers used for tumors; PCNA, Ki-67, CDs and others. | Reading lecture notes, Scanning the internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Beyin Fırtınası, Gösteri |
| 11 | In immunocytochemical and immunocytochemical methods, General biomarkers for tumors of epithelial origin; Pan-Cytokeratin, PCNA, Ki-67, S100, CDs and others. | Reading lecture notes, Scanning the internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Beyin Fırtınası, Gösteri |
| 12 | In immunocytochemical and immunocytochemical methods, general biomarkers used for tumors of mesenchymal origin; Vimentin, Desmin, SMA, CDs and others. | Reading lecture notes, Reviewing visual materials | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Beyin Fırtınası, Gösteri, Alıştırma ve Uygulama |
| 13 | Application of immunocytochemical method to tissue and smears in laboratory environment. | Reviewing the ISC method in the lab | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Problem Çözme, Deney / Laboratuvar, Alıştırma ve Uygulama |
| 14 | Application of immunohistochemical method to tissues in laboratory environment. | Reviewing the IHC method in the lab | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Problem Çözme, Beyin Fırtınası, Deney / Laboratuvar |
| 15 | Microscopic examination, evaluation and reporting of Immunohistochemistry and Immunocytochemistry preparations prepared in laboratory environment | Reviewing sample cases, Preparing for the lab | Öğretim Yöntemleri: Tartışma, Alıştırma ve Uygulama, Beyin Fırtınası, Gösterip Yaptırma |
| 16 | Term Exams | Preparing for the exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Performans Değerlendirmesi, Ödev |
| 17 | Term Exams | Preparing for the exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Performans Değerlendirmesi, Ödev |
Assessment (Exam) Methods and Criteria
| Assessment Type | Midterm / Year Impact | End of Term / End of Year Impact |
|---|---|---|
| 1. Midterm Exam | 100 | 40 |
| General Assessment | ||
| Midterm / Year Total | 100 | 40 |
| 1. Final Exam | - | 60 |
| Grand Total | - | 100 |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 6 | 84 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 12 | 12 |
| Total Workload (Hour) | 162 | ||
| Total Workload / 25 (h) | 6,48 | ||
| ECTS | 6 ECTS | ||