TÜRKÇE
ENGLISH
Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (PhD) (INTERDISCIPLINARY) | |
| Code | BT0088 |
| Name | Transgenic Animal Technology and in vivo Modeling |
| Term | 2024-2025 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
Dr. Öğr. Üyesi SİNAN KANDIR
(Bahar)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
The aim of this course is to teach transgenic animal technologies and genetic engineering methods, explain in vivo model development processes in experimental research, introduce assisted reproductive technologies and genetic modification processes, evaluate animal experiments within the framework of ethics and the 3R principle, and provide knowledge on the production and utilization of transgenic animals through biotechnological approaches.
Course Content
Transgenic animal production, products and technologies, embryology, superovulation, intracytoplasmic sperm injection, microinjection, somatic cell transfer (cloning), retrovirus mediated gene transfer, embryonic stem cell mediated gene transfer, next generation gene editing tools and their uses, transgenic animal by research topic design (selection of bovine, ovine, poultry, laboratory animals).
Course Precondition
N/A
Resources
1) Transgenic Animal Technology, Elsevier (2014). 2) Genetic engineering in livestock: New applications and interdisciplinary perspectives, Springer-verlag Heidelberg. (2009) 3) Transgenic Animals in Agriculture, CABI. (1994) and related literature
Notes
1) Transgenic Animal Technology, Elsevier (2014). 2) Genetic engineering in livestock: New applications and interdisciplinary perspectives, Springer-verlag Heidelberg. (2009) 3) Transgenic Animals in Agriculture, CABI. (1994) and related literature
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explains the processes of transgenic animal production. |
| LO02 | Defines assisted reproductive techniques used in experimental animals. |
| LO03 | Learns to create specific animal models using genetic engineering tools. |
| LO04 | Compares gene transfer methods used in transgenic animal technologies. |
| LO05 | Applies in vivo model development processes for experimental research. |
| LO06 | Evaluates ethical principles and the 3R principle in animal experiments. |
| LO07 | Explains the significance of transgenic animal applications in agriculture, healthcare, and biomedical research. |
| LO08 | Applies genetic modification processes using biotechnological methods. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops current and advanced knowledge in the field at the level of expertise with original thought and/or research, based on graduate qualifications. | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | 3 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | 5 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | 3 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | 4 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | 5 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | 3 |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to transgenic animal models | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | Application areas of transgenic animals | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Grup Çalışması |
| 3 | Gene transfer techniques used in the production of transgenic animals | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | Control of estrus and superovulation | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | Assisted reproductive techniques | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | Genetic replication and its importance in animal production | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma |
| 7 | artificial insemination; advantages and disadvantages | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap |
| 8 | Gene transfers (Micro-injection, use of embryonic stem cells, retroviral vectors, other gene transfer methods) | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 9 | Gene transmissions (establishment of transgenic lines, breeding programs with transgenic animals) | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Mouse / mice (disease models) | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 11 | Rats (disease models) | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 12 | Transgenic applications in farm / large animals | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma |
| 13 | Applications in farm / large animals | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Transgenic applications in poultry | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | Transgenic applications in aquaculture animals | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Transgenic organisms and ethics | Related resources, lecture notes, and literature | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Proje / Tasarım, Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 5 | 70 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 4 | 8 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 4 | 4 |
| Final Exam | 1 | 10 | 10 |
| Total Workload (Hour) | 162 | ||
| Total Workload / 25 (h) | 6,48 | ||
| ECTS | 6 ECTS | ||