TÜRKÇE
ENGLISH
Information
| Code | BT176 |
| Name | Methods Used in Molecular Epidemiology |
| Term | 2024-2025 Academic Year |
| Term | Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. TÜLİN GÜVEN GÖKMEN |
| Course Instructor |
Doç. Dr. TÜLİN GÜVEN GÖKMEN
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
Within the scope of molecular epidemiology, it is aimed for the student to explain the ways of identifying infectious diseases caused by various microorganisms (bacteria, viruses, fungi, etc.) using molecular methods, to know the methods to detect their source, to provide information about their spread, transmission routes, pathogenesis, detection of virulence and resistance genes, and vaccine production.
Course Content
The definition of Molecular Epidemiology includes topics related to its field of use and which methods will be used in the identification of infection, epidemics and pandemics.
Course Precondition
None
Resources
Ders Notları Molecular Epidemiology: A Practical Approach M. Carrington and A.R. Hoelzel, ed., Oxford University Press,
Notes
Image sources Current articles
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Describe epidemiology and its importance in society. |
| LO02 | Explains the importance of molecular epidemiology in the control of infections. |
| LO03 | Explains the definition of Molecular Epidemiology and why it is necessary |
| LO04 | Lists Molecular Epidemiological methods. |
| LO05 | Chooses when Molecular Epidemiological methods should be used. |
| LO06 | Explains Molecular Epidemiological methods in detail. |
| LO07 | Selects software that is suitable for the methods used in molecular epidemiology. |
| LO08 | Explains which molecular epidemiological method should be chosen for the agent |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develops current and advanced knowledge in the field at the level of expertise with original thought and/or research, based on graduate qualifications. | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | 4 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | 5 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. | 2 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | What is Molecular Epidemiology? | None preparation | Öğretim Yöntemleri: Anlatım |
| 2 | Introduction to Molecular Epidemiology When is it used? How to use? Where does its border begin and end? | Reading lecture notes and current articles | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma |
| 3 | Molecular Microbiology Laboratory Why is it necessary? What is needed? How should the design of the laboratory be? What are the necessary biosecurity measures? | Reading lecture notes, Reviewing visual materials | Öğretim Yöntemleri: Anlatım, Tartışma, Beyin Fırtınası |
| 4 | Nucleic Acid Isolation | Reading books and watching videos | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 5 | Knowledge of Nucleic Acid Isolation Applications | Reading lecture notes and books | Öğretim Yöntemleri: Anlatım, Tartışma, Benzetim |
| 6 | Polymerase chain reaction and its types Transcription-mediated amplification | Reading lecture notes, Reviewing visual materials | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 7 | Nucleic acid amplification methods Loop-mediated amplification | Reading lecture notes, watching videos | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma, Benzetim |
| 8 | Mid-Term Exam | Exam preparation | Ölçme Yöntemleri: Sözlü Sınav, Yazılı Sınav |
| 9 | Arbitrarily primed PCR (AP-PCR) - Restriction length polymorphism (PCR-RFLP) | Reading Course notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | Nucleic acid amplification based molecular typing methods REP-PCR ERIC-PCR | Reading book | Öğretim Yöntemleri: Anlatım, Tartışma, Grup Çalışması |
| 11 | Nucleic acid amplification based molecular typing methods -Multilokus Variable Number Tandem Repeat (MLVA) | Reading books and watching videos | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Benzetim |
| 12 | Sequencing based molecular typing methods -MLST -Spa typing | Reading lecture notes, Reviewing visual materials, Reading current articles | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Beyin Fırtınası |
| 13 | Hybridization-based molecular typing methods -Spoligotyping | Reading current articles | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Pulsed Field Gel Electrophoresis (PFGE) | Reviewing visual materials | Öğretim Yöntemleri: Anlatım, Tartışma |
| 15 | Pulsed Field Gel Electrophoresis (PFGE) in epidemic analysis | Reading lecture notes and current articles | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Anlatım, Örnek Olay |
| 16 | Term Exams | Exam preparation | Ölçme Yöntemleri: Sözlü Sınav, Yazılı Sınav |
| 17 | Term Exams | Exam preparation | Ölçme Yöntemleri: Sözlü Sınav, Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 7 | 98 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 1 | 1 |
| Final Exam | 1 | 1 | 1 |
| Total Workload (Hour) | 142 | ||
| Total Workload / 25 (h) | 5,68 | ||
| ECTS | 6 ECTS | ||