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Information
| Code | BT176 |
| Name | Methods Used in Molecular Epidemiology |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. TÜLİN GÜVEN GÖKMEN |
Course Goal / Objective
Molecular methods within the scope of molecular epidemiology are intended to provide information about the identification of infectious diseases caused by various microorganisms (bacteria, viruses, fungi, etc.), determination of their source, spread, transmission routes, pathogenesis, detection of virulence and resistance genes, and vaccine production.
Course Content
It includes the identification of infectious diseases caused by various microorganisms (bacteria, viruses, fungi, etc.), determination of their source, spread, transmission routes, pathogenesis, detection of virulence and resistance genes, and vaccine production.
Course Precondition
None
Resources
Ders Notları
Notes
Molecular Epidemiology: A Practical Approach M. Carrington and A.R. Hoelzel, ed., Oxford University Press,
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Chooses when Molecular Epidemiological methods should be used. |
| LO02 | Explains the definition of Molecular Epidemiology and why it is necessary |
| LO03 | Lists Molecular Epidemiological methods. |
| LO04 | Defines Molecular Epidemiological methods |
| LO05 | Selects software that is suitable for the methods used in molecular epidemiology. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develop and deepen the current and advanced knowledge in the field with original thought and / or research at the level of expertise based on the qualifications of the graduate and reach the original definitions that will bring innovation to the field. | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | 4 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | 5 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | What is Molecular Epidemiology? | Lecture note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma |
| 2 | Introduction to Molecular Epidemiology When is it used? How to use? Where does its border begin and end? | Lectıre note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma |
| 3 | Molecular Microbiology Laboratory Why is it necessary? What is needed? How should the design of the laboratory be? What are the necessary biosecurity measures? | Lecture note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma |
| 4 | Nucleic Acid Isolation | Lecture note | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 5 | Nucleic Acid Isolation Applications | Lecture note | Öğretim Yöntemleri: Deney / Laboratuvar |
| 6 | Polymerase chain reaction and its types Transcription-mediated amplification | Lecture note | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma, Deney / Laboratuvar |
| 7 | Nucleic acid amplification methods Loop-mediated amplification | Lecture Note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Exam preparing | Ölçme Yöntemleri: Sözlü Sınav |
| 9 | Arbitrarily primed PCR (AP-PCR) - Restriction length polymorphism (PCR-RFLP) | Lecture note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma, Deney / Laboratuvar |
| 10 | Nucleic acid amplification based molecular typing methods -Repetitive extragenic palindromic (REP)-PCR - Enterobacterial repetitive intergenic consensus (ERIC)-PCR | Lecture Note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma, Deney / Laboratuvar |
| 11 | Nucleic acid amplification based molecular typing methods -Multilokus Variable Number Tandem Repeat (MLVA) | Lecture Note | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Anlatım, Deney / Laboratuvar |
| 12 | Sequencing based molecular typing methods -MLST -Spa typing | Lecture Note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma, Deney / Laboratuvar |
| 13 | Hybridization-based molecular typing methods -Spoligotyping | Lecture Note | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap, Deney / Laboratuvar |
| 14 | Pulsed Field Gel Electrophoresis (PFGE) in epidemic analysis 1 | Lecture Note | Öğretim Yöntemleri: Soru-Cevap, Anlatım, Tartışma, Deney / Laboratuvar |
| 15 | Pulsed Field Gel Electrophoresis (PFGE) in epidemic analysis 2 | Lecture Note | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Anlatım, Deney / Laboratuvar |
| 16 | Term Exams | Exam preparing | Ölçme Yöntemleri: Sözlü Sınav |
| 17 | Term Exams | Exam preparing | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||