TÜRKÇE
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Information
| Code | SY028 |
| Name | Gastrointestinal Microbiota and Analysis Methods in Fish |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Miray ETYEMEZ BÜYÜKDEVECİ |
Course Goal / Objective
It is aimed to provide detailed information about the gastrointestinal system in fish, to provide information about the microorganisms in the gastrointestinal microbiota and their functional tasks, to examine the role of microbiota on disease factors in terms of fish health and to evaluate the methods used in the identification of microbiota.
Course Content
Definition of microbiota, Structure of the digestive system in fish, Differences in intestinal structure depending on nutrition, Microorganism groups in gut microbiota of fish, Factors affecting fish gut microbiota, Physiology of fish gut microbiome and its role in immunity, Function of gut microbiota in nutrition, The role of gut microbiota in fish diseases, Methods used in the manipulation of gut microbiota, Metagenomic studies of fish gut microbiome, Determination of gut microbial communities of fish by 16S rRNA gene sequence analysis, Examination of current research on the evaluation of gut microbiota in fish
Course Precondition
None
Resources
Lecture Notes Romero, J., Ringø, E., & Merrifield, D. L. (2014). The gut microbiota of fish. Aquaculture nutrition: Gut health, probiotics and prebiotics, 75-100. Yukgehnaish, K., Kumar, P., Sivachandran, P., Marimuthu, K., Arshad, A., Paray, B. A., & Arockiaraj, J. (2020). Gut microbiota metagenomics in aquaculture: factors influencing gut microbiome and its physiological role in fish. Reviews in Aquaculture, 12(3), 1903-1927. López Nadal, A., Ikeda-Ohtsubo, W., Sipkema, D., Peggs, D., McGurk, C., Forlenza, M., ... & Brugman, S. (2020). Feed, microbiota, and gut immunity: using the zebrafish model to understand fish health. Frontiers in Immunology, 11, 512428. Pérez, T., Balcázar, J. L., Ruiz-Zarzuela, I., Halaihel, N., Vendrell, D., de Blas, I., & Múzquiz, J. L. (2010). Host–microbiota interactions within the fish intestinal ecosystem. Mucosal immunology, 3(4), 355-360.
Notes
recent articles
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Gains basic knowledge of microorganism groups in microbiota |
| LO02 | Defines the concept of gastrointestinal microbiota in fish |
| LO03 | Gains knowledge of factors affecting gut microbiota in fish |
| LO04 | Gains the ability to apply DNA isolation protocol from fish intestine |
| LO05 | Explain the basic principles of next generation DNA sequencing methods used in the analysis of intestinal microbiota. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Improves and deepens the institutional and practical knowledge in Aquaculture and Diseases | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehend the interdisciplinary interaction related to the field of expertise | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Synthesizes and produces new theories with theoretical and practical knowledge specialized in Aquaculture and Diseases Departments. | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | It collects data about the subjects that it specializes in Aquaculture and Diseases Departments, evaluates the results, establishes cause and effect relationship and produces solutions. | 3 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Plans and conducts a study that requires expertise in Aquaculture and Diseases alone. It contributes to the solution of the problems that may occur as a specialist or as an individual or ancillary researcher and takes responsibility. | 2 |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | It reaches and uses resources related to Aquaculture and Diseases. | 2 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transmits the developments related to Aquaculture and Diseases in written, oral and visual form. | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | He / she establishes written and verbal communication about the subject that he / she specializes in and he / she follows the meetings and seminars. | 2 |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | She/He develops strategy, policy and implementation plans in the fields of Aquaculture and Diseases, of which she/he is an expert, and evaluates the results obtained within the framework of total quality management processes. Uses the knowledge and experience gained in the fields of Aquaculture and Diseases, problem solving and/or application skills in interdisciplinary studies. | 2 |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Develops a positive attitude towards lifelong learning. | |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Gains the competence to develop plans and design proposals sensitive to society, space and nature for different types of production. | 2 |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | She/he gains the ability to develop and deepen her knowledge in the fields of Aquaculture and Diseases, in which she/he is an expert. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Definition of the microbiota | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | The structure of the digestive system in fish, differences in intestinal structure depending on nutrition | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 3 | Membership of gut microbial communities in fish | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | Factors affecting fish gut microbiota | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 5 | Physiology of the fish gut microbiome and its role in immunity | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 6 | The function of gut microbiota in nutrition | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 7 | Midterm homework , general repetition | Studying for exam | Öğretim Yöntemleri: Anlatım, Tartışma |
| 8 | Mid-Term Exam | Studying for exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | The role of gut microbiota in fish disease | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | Methods used in the manipulation of intestinal microbiota; Probiotics | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 11 | Metagenomic studies of the fish gut microbiome | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 12 | Determination of gut microbial communities of fish by 16S rRNA gene sequence analysis | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 13 | Assessment of current research about gut microbiota in fish | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | DNA isolation method from intestinal tissue | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 15 | General repetition | Studying for exam | Öğretim Yöntemleri: Anlatım, Tartışma |
| 16 | Term Exams | Studying for exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Studying for exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 2 | 15 | 30 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 10 | 10 |
| Final Exam | 1 | 10 | 10 |
| Total Workload (Hour) | 162 | ||
| Total Workload / 25 (h) | 6,48 | ||
| ECTS | 6 ECTS | ||