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Information
| Code | SY031 |
| Name | Basic Molecular Biology Techniques |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Serdar KİLERCİOĞLU |
Course Goal / Objective
This course aims to convey and practically teach theoretical knowledge of molecular biology techniques used for various purposes such as diagnosis, establishing cause-effect relationships, and understanding mechanisms in different scientific fields.
Course Content
In this course, the structure and functions of biomolecules that make up living things will be discussed specifically for aquatic creatures. DNA, RNA, gene, chromosome, cell, etc. Definitions will be discussed and their functions will be learned. In addition, formation processes such as information transfer from DNA to DNA and from DNA to RNA, nucleotide language, amino acid language will be covered. Additionally, applications such as RNA and DNA isolation, cDNA synthesis, target region amplification and gene expression will be learned practically in the laboratory environment.
Course Precondition
NONE
Resources
Turner, P. C., McLeman A. G., Bates, A. D., White M. R. H. (2004). Molecular Biology Important Notes. Nobel Publisher, 346 page. Yıldırım, A., Bardakçı, F., Karataş, M., Tanyolaç, B. (2010). Moleküler Biyoloji. Nobel Yayın Dağıtım. 2. baskı,685 syf.
Notes
Lecture Notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Students comprehends knowledges such as molecular biology, gene, dna, rna |
| LO02 | Learns the basic application techniques |
| LO03 | Learns the things about the laboratory and the working order, and also learns the use of relevant laboratory equipment and devices |
| LO04 | Becomes capable of doing basic research |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Improves and deepens the institutional and practical knowledge in Aquaculture and Diseases | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehend the interdisciplinary interaction related to the field of expertise | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Synthesizes and produces new theories with theoretical and practical knowledge specialized in Aquaculture and Diseases Departments. | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | It collects data about the subjects that it specializes in Aquaculture and Diseases Departments, evaluates the results, establishes cause and effect relationship and produces solutions. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Plans and conducts a study that requires expertise in Aquaculture and Diseases alone. It contributes to the solution of the problems that may occur as a specialist or as an individual or ancillary researcher and takes responsibility. | |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | It reaches and uses resources related to Aquaculture and Diseases. | |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Transmits the developments related to Aquaculture and Diseases in written, oral and visual form. | |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | He / she establishes written and verbal communication about the subject that he / she specializes in and he / she follows the meetings and seminars. | 2 |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | She/He develops strategy, policy and implementation plans in the fields of Aquaculture and Diseases, of which she/he is an expert, and evaluates the results obtained within the framework of total quality management processes. Uses the knowledge and experience gained in the fields of Aquaculture and Diseases, problem solving and/or application skills in interdisciplinary studies. | |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Develops a positive attitude towards lifelong learning. | 2 |
| PLO11 | Yetkinlikler - Alana Özgü Yetkinlik | Gains the competence to develop plans and design proposals sensitive to society, space and nature for different types of production. | 2 |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | She/he gains the ability to develop and deepen her knowledge in the fields of Aquaculture and Diseases, in which she/he is an expert. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Biomolecules, Chemical bonds | Reading of course subject | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 2 | Biomolecule classifications (Nucleotides, amino acids, fatty acids, carbohydrates) | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 3 | Transition from cell to organism | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 4 | Nucleic acids and their properties | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 5 | Gene and genome | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 6 | Transcription regulation | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 7 | Structure and functions of proteins | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Gösteri, Tartışma |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Laboratory pre-application | Transforming the acquired knowledge into practice | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma, Deney / Laboratuvar |
| 10 | Laboratory application 1 | Transforming the acquired knowledge into practice 1 | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma, Deney / Laboratuvar |
| 11 | Laboratory application 2 | Transforming the acquired knowledge into practice 2 | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma, Deney / Laboratuvar |
| 12 | Laboratory application 3 | Transforming the acquired knowledge into practice 3 | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma, Deney / Laboratuvar |
| 13 | Homework | Reading the lecture notes and related resources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Laboratory application 4 | Transforming the acquired knowledge into practice 4 | Öğretim Yöntemleri: Grup Çalışması, Deney / Laboratuvar, Soru-Cevap |
| 15 | Laboratory application 5 | Transforming the acquired knowledge into practice 5 | Öğretim Yöntemleri: Tartışma, Grup Çalışması, Deney / Laboratuvar, Soru-Cevap |
| 16 | Term Exams | Studying for exam | Ölçme Yöntemleri: Sözlü Sınav, Ödev |
| 17 | Term Exams | Studying for exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 21 | 21 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 10 | 10 |
| Final Exam | 1 | 10 | 10 |
| Total Workload (Hour) | 153 | ||
| Total Workload / 25 (h) | 6,12 | ||
| ECTS | 6 ECTS | ||