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Information
| Unit | CEYHAN FACULTY OF VETERINARY |
| Veterinary PR. | |
| Code | VTS214 |
| Name | Analytical Methods in Pharmacology and Toxicology |
| Term | 2024-2025 Academic Year |
| Semester | 4. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Lisans Dersi |
| Type | Normal |
| Label | VK Vocational Knowledge Courses E Elective |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. PETEK PİNER BENLİ |
| Course Instructor |
Prof. Dr. PETEK PİNER BENLİ
(Bahar)
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
It is aimed to reveal the presence and amount of drugs/poisons that affect body systems and to learn the laboratory devices used for this purpose and their working principles theoretically and practically.
Course Content
It includes basic laboratory tools and equipment and their use, working principles, use and working principle of spectrophotometer, chromatographic analysis and its types, sample preparation stages, genotoxicity and its measurement.
Course Precondition
None
Resources
İlaç ve Zehir Laboratuvar Uygulama Kitabı, Abdullah Doğan, 2019
Notes
Kromatografik Yöntemler, Ayla Demirci, Mustafa Özcimder, 2019. Enstrümantal Analiz İlkeleri, Esma Kılıç, Hamza Yılmaz, 2013 Zehirli Maddelerin Laboratuvar Analizi, Sezai Kaya, 2012
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Expresses basic laboratory tools and equipment, their use and working principles. |
| LO02 | Explains the use and working principle of spectrophotometry. |
| LO03 | List the basic principles used in chromatographic analysis. |
| LO04 | Summarizes the purpose for which Atomic Absorption Spectroscopy technique is used and its application method. |
| LO05 | List the stages of fluorescence spectroscopy technique used in drug analysis. |
| LO06 | Explain sample preparation methods in chromatographic analysis. |
| LO07 | Express how to use HPCL technique in pharmacological and toxicological analysis. |
| LO08 | Exemplifies how to make qualitative and quantitative analyzes of drugs and toxic substances using GC technique. |
| LO09 | Compare the sections of HPLC and GC devices in terms of their similarities and differences. |
| LO10 | Exemplifies the application areas of Thin Layer Chromatography technique in pharmacology and toxicology with examples. |
| LO11 | List the stages of genotoxicity tests and the Comet method. |
| LO12 | Express the stages of ELISA Technique and how it is applied. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Distinguishes animal species and breeds based on their structural (morphological), functional (physiological, biochemical) and behavioral characteristics and whether they can be consumed for food purposes. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Explains the sources, physical and chemical properties and preparation of drugs. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Describes the effects and side effects of medications on patients | 3 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Explains the formation mechanisms of animal diseases and their pathological effects on the body. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | It makes efficiency evaluations among animal breeds and, when necessary, carries out selection, hybridization and artificial insemination at a level that can carry out breeding work. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Disposes of dead animal material, tissue samples, medical and chemical waste appropriately in accordance with international, national laws and regulations. | |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | It determines the correct approach area for the patient by evaluating the examination, operation etc. practices in the clinic according to the topographic anatomy. | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Uses biochemical parameters in diagnostic processes by evaluating the information obtained with cause-effect relationships. | |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | Writes prescriptions in accordance with diagnosis and treatment protocols for diseases and poisoning in animals. | 3 |
| PLO10 | Beceriler - Bilişsel, Uygulamalı | Implements necessary measures, including biosecurity, to control infectious diseases. | |
| PLO11 | Beceriler - Bilişsel, Uygulamalı | Performs necropsy to evaluate findings in dead animals, directs tissues to the necessary laboratory according to appropriate storage methods and prepares a report. | |
| PLO12 | Beceriler - Bilişsel, Uygulamalı | Monitors patients in the post-operative process according to the main treatment methods of surgical diseases in animal species. | |
| PLO13 | Beceriler - Bilişsel, Uygulamalı | Applies sedation, anesthesia and pain management methods safely according to animal species. | |
| PLO14 | Beceriler - Bilişsel, Uygulamalı | Correctly applies biosafety principles such as sterilization of clinical and laboratory equipment and clothing. | 3 |
| PLO15 | Beceriler - Bilişsel, Uygulamalı | It regulates the care and feeding protocols according to animal species and supervises the consumption of animal foods produced for human consumption or foods containing animal products in their composition. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Evaluates laboratory results of animal diseases and applies correct diagnosis, prognosis and treatment methods based on the data obtained. | |
| PLO17 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | He/she is conscious of performing his/her profession by being aware of his/her powers and responsibilities. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Examines scientific techniques and methods according to facts related to veterinary services and takes analysis or methodological responsibility for problems. | 3 |
| PLO19 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It uses adequate technology in the hygienic production, manufacturing, processing, consumption and sales of food by conducting health control of animal food products produced for human consumption. | |
| PLO20 | Yetkinlikler - Öğrenme Yetkinliği | Lists the methods of producing and using scientific knowledge in his/her professional field. | |
| PLO21 | Yetkinlikler - Öğrenme Yetkinliği | Uses updated legislation regarding veterinary services when necessary. | |
| PLO22 | Yetkinlikler - Öğrenme Yetkinliği | Performs an examination appropriate to the case, applying the contribution of imaging methods to diagnosis and radiation safety in accordance with current regulations. | |
| PLO23 | Yetkinlikler - Öğrenme Yetkinliği | Keeps clinical and patient records using proper and scientific methods. | |
| PLO24 | Yetkinlikler - Öğrenme Yetkinliği | By observing the basic principles of medicine, it develops management methods and appropriate management plans for animal production enterprises. | |
| PLO25 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Implement social projects and plans with social awareness, based on professional skills and authority with a computer usage license, advanced computer software. | |
| PLO26 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Works in cooperation with other colleagues by following the world agenda within the framework of professional ethics and deontology rules. | 2 |
| PLO27 | Yetkinlikler - Alana Özgü Yetkinlik | Produces solutions to field-specific problems in line with scientific data/evidence. | |
| PLO28 | Yetkinlikler - Alana Özgü Yetkinlik | He/she has the ethical values of the veterinary profession and defends these values. | |
| PLO29 | Yetkinlikler - Alana Özgü Yetkinlik | Takes appropriate precautions by clinically recognizing zoonosis and notifiable diseases. | |
| PLO30 | Yetkinlikler - Alana Özgü Yetkinlik | Determines the methods of diagnosis, prevention, treatment and operation of diseases in different animal species. | |
| PLO31 | Yetkinlikler - Alana Özgü Yetkinlik | In emergency cases, intervenes in all animals and applies first aid methods. | |
| PLO32 | Yetkinlikler - Alana Özgü Yetkinlik | Makes judgments on animal husbandry principles and breeding by assessing the physical condition, welfare and rearing conditions of domestic animals, communicating appropriately with animal owners and colleagues. | |
| PLO33 | Yetkinlikler - Alana Özgü Yetkinlik | Independently interprets articles and presentations published in other scientific fields related to Veterinary Medicine to maintain professional development and skills. | 3 |
| PLO34 | Yetkinlikler - Alana Özgü Yetkinlik | When necessary, euthanizes animals using appropriate methods and respecting the feelings of animal owners. | |
| PLO35 | Yetkinlikler - Alana Özgü Yetkinlik | By mastering the concepts of occupational safety in terms of the workplace and the personnel working with it, they have the competence to apply and defend it. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction, Basic laboratory safety procedures, Basic laboratory tools and equipment | No pre-preparation | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 2 | Spectrophotometric Methods | Reading lecture notes, reviewing visual data, reviewing current articles | Öğretim Yöntemleri: Anlatım |
| 3 | Atomic Absorption Spectroscopy | Reading lecture notes, reviewing visual data, reviewing current articles, researching on the internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 4 | Fluorescence and Phosphorescence Spectroscopy | Reading lecture notes, researching on the internet | Öğretim Yöntemleri: Tartışma |
| 5 | Basic Principles of Chromatography | Reading lecture notes, researching on the internet, reviewing visual data | Öğretim Yöntemleri: Soru-Cevap |
| 6 | Thin Layer Chromatography (TLC) -I | Reading lecture notes, reading textbook, reviewing visual data | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 7 | Thin Layer Chromatography (TLC) -II | Reading lecture notes, researching on the internet | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | High Performance Liquid Chromatography (HPLC) -I | Reading lecture notes, reviewing visual data, reviewing current articles, researching on the internet | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | High Performance Liquid Chromatography (HPLC) -II | Reading lecture notes, reviewing visual data, reviewing current articles | Öğretim Yöntemleri: Anlatım |
| 11 | Gas Chromatography (GC) -I | Reading lecture notes, reviewing visual data | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Gas Chromatography (GC) -II | Reading lecture notes, reviewing visual data, reviewing current articles | Öğretim Yöntemleri: Anlatım |
| 13 | Comet Assay-I | Reading lecture notes, reading textbook, doing assignment | Öğretim Yöntemleri: Soru-Cevap |
| 14 | Comet Assay-II | Reading lecture notes, reading textbook, doing assignment | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | ELISA | Reading lecture notes, reading textbook | Öğretim Yöntemleri: Soru-Cevap |
| 16 | Term Exams | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Exam preparation | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 4 | 4 |
| Total Workload (Hour) | 76 | ||
| Total Workload / 25 (h) | 3,04 | ||
| ECTS | 3 ECTS | ||