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Information
| Code | KMB507 |
| Name | Molecular Microbiology |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. FATİH KÖKSAL |
Course Goal / Objective
To create awareness about microorganisms and organisms studied within the scope of microbiology. To emphasize the importance of signal transmission by showing the relationships of these organisms with other organisms and the environment. To increase the level of knowledge about microorganism metabolism and reproduction. To provide general information about diseases caused by microorganisms and molecular methods used in diagnosis.
Course Content
Diseases caused by microorganisms and ways to combat them. Molecular methods used in microbiological diagnosis.
Course Precondition
To have general knowledge about the basic concepts of microbiology
Resources
Brock Biology of Microorganisms (Eds. Madigan M.T., Martinko J.M., Parker J.) Microbiology (Eds. Prescott L.M., Harley J.P., Klein D.A.) Molecular Microbiology: Diagnostic Principles and Practice (ASM Books) 3rd.Edition (Eds: Persing DH, Tenover FC, Versalovic J, Tang Y, Unger ER, Relman DA, White TJ). ASM Press, Washington DC, 2016.
Notes
Google scholar, pubmed etc.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Have knowledge about DNA sequence analysis methods |
| LO02 | Defines nucleic acid replication and detection methods |
| LO03 | Can express mutation detection methods and their place in the clinic |
| LO04 | Apply agarose gel electrophoresis methods |
| LO05 | Learn the principles of safe working in molecular microbiology laboratory |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To have up to date knowledge at the level of expertise in the field of health based on undergraduate level qualifications, to develop and deepen them. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To have knowledge about statistics as a tool that is constantly used in studies in the field of health, to use related software effectively. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Uses the theoretical and practical knowledge at the level of expertise in the field of health. | 1 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Integrates the knowledge in the field of health with information from different disciplines and interprets it to create new information, analyzes and synthesizes using different research methods and proposes solutions. | 3 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Writes the report of his/her research. | 4 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Carries out studies related to the field of health independently and / or as a team. | 2 |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Publishes a scientific article in a national journal or presents it in a scientific meeting. | 5 |
| PLO08 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Conducts scientific clinical and/or descriptive research/presentation/publication on priority issues related to the field and public health | 3 |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Evaluates the information related to the field of health with a critical approach and directs his/her learning. | |
| PLO10 | Yetkinlikler - Öğrenme Yetkinliği | To be able to apply the principles of professional development and lifelong learning in the field of health in his/her studies. | |
| PLO11 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To be able to systematically discuss and share their knowledge, current developments and their own work in the field of health with groups in the same field or outside the same field in written, oral and visual ways. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | The place of molecular techniques in microbiology | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Physical structure of the molecular microbiology laboratory | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 3 | Cell lysis and nucleic acid purification methods | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | Nucleic acid amplification and detection methods | Course resources and book | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 5 | DNA Sequence Analysis | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 6 | Electropheresis | Course resources and book | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 7 | Molecular typing methods | Course resources and book | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 8 | Exam | Course resources and book | Ölçme Yöntemleri: Sözlü Sınav |
| 9 | Molecular epidemiology | Course resources and book | Öğretim Yöntemleri: Anlatım |
| 10 | Mutation detection methods | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 11 | Plasmid analysis | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 12 | Cloning methods | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 13 | Use of molecular methods in diagnosis and standardization | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 14 | Laboratory safety | TCourse resources and book | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 15 | Student presentation | Course resources and book | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma |
| 16 | Exam | Course resources and book | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Exam | Course resources and book | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 16 | 16 |
| Total Workload (Hour) | 108 | ||
| Total Workload / 25 (h) | 4,32 | ||
| ECTS | 4 ECTS | ||