TÜRKÇE
ENGLISH
Information
| Code | MİK612 |
| Name | Molecular Identification Methods of Viral Infections |
| Term | 2024-2025 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. FÜGEN YARKIN |
| Course Instructor |
Prof. Dr. FÜGEN YARKIN
(A Group)
(Ins. in Charge)
|
Course Goal / Objective
To teach the importance and application areas of molecular methods used in the diagnosis and identification of viruses
Course Content
Viral infections and diagnostic methods, molecular biology basics, polymerase chain reaction (PCR) methods, sequence analysis and genome analysis, rapid diagnostic methods and clinical applications of diagnostic methods
Course Precondition
Basic microbiology knowledge is important to understand the structure, replication and pathogenesis of viruses. Having basic knowledge on topics such as DNA and RNA structure, gene expression. Basic knowledge on enzymes and biomolecules contributes to the understanding of molecular techniques.
Resources
Flint SJ et al. Principles of Virology Second Edition, ASM Press. 2004
Notes
Us D.,Ergünay K. MOLECULAR,Clinical and Diagnostic Virology.Scientific Medicine. 2012
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Define PCR and explain PCR types |
| LO02 | Have general knowledge about viral infection and diagnosis |
| LO03 | Learns and explains genome sequencing and analysis |
| LO04 | Understands nucleic acid based tests |
| LO05 | Count molecular methods in the diagnosis of viral infection and give general information |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Beceriler - Bilişsel, Uygulamalı | Having acquired technical skills through laboratory studies, being able to access information, conduct research, plan experiments, solve problems, interpret experimental results, make presentations, develop strategies on microbiology-related issues | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Having received a quality microbiology education at a standard accepted in international platforms, having broadened scientific horizons and being preferred in many sectors related to microbiology, being competent in microbiology | 2 |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Isolates infectious agents | 3 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Performs the steps of identifying infectious agents | |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Knowing and applying laboratory methods used in general bacteriology, virology, parasitology, mycology and immunology at theoretical and practical levels | 4 |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Being aware of ethical values and problems in the field of microbiology | |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Ability to make critical evaluations on published research in the field of microbiology | 2 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Gaining the competence to present a research conducted in the field of microbiology in written, oral and visual form and prepare it for publication | 3 |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Having acquired research and career ethics | 1 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Having deepened knowledge on specific subjects studied (thesis study) | 5 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General characteristics of viral infections | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Traditional diagnostic methods and their limitations | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 3 | Molecular Biology Fundamentals | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 4 | DNA and RNA viruses | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 5 | Polymerase Chain Reaction (PCR) Methods | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 6 | Real-time PCR | Course resources and book | Öğretim Yöntemleri: Anlatım, Tartışma |
| 7 | Nested PCR | Course resources and book | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 8 | Exam | Course resources and book | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Reverse Transcription PCR (RT-PCR) | Course resources and book | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 10 | Sequencing and Genome Analysis | Course resources and book | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 11 | Sorting techniques | Course resources and book | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Genome sequencing and analysis | Course resources and book | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 13 | Identification of viral variants | Course resources and book | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 14 | Rapid Diagnostic Methods | Course resources and book | Öğretim Yöntemleri: Anlatım, Deney / Laboratuvar |
| 15 | Antigen diagnostic tests | Course resources and book | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 16 | Nucleic acid-based tests | Course resources and book | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 17 | Exam | Course resources and book | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 7 | 7 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 109 | ||
| Total Workload / 25 (h) | 4,36 | ||
| ECTS | 4 ECTS | ||