FAR699 invivo methods in evaluation of drug effects

Information

Course Goal / Objective

The aim of this course is to provide students with a comprehensive understanding of in vivo methods used to evaluate the effects of drugs on living organisms and to examine in detail the basic principles, application areas, advantages and limitations of these methods.

Course Content

Introduction (definition and importance of in vivo studies, the place of in vivo studies in the drug development process), Ethical principles of in vivo studies and animal welfare, 3R principle (Replacement, Reduction, Refinement) in in vivo studies, In vivo study design (Objective and hypothesis determination, experimental animal selection (species, gender, age, genetic characteristics, etc.), control groups and application methods, experimental protocol creation (dose, duration, route of administration, etc.), data collection and analysis methods), In Vivo Methods (Pharmacokinetic studies (absorption, distribution, metabolism, etc.) ), control groups and application methods, creating an experimental protocol (dose, duration, route of administration, etc.), data collection and analysis methods), In Vivo Methods (Pharmacokinetic studies (absorption, distribution, metabolism, elimination), determination of pharmacokinetic parameters (area under the curve (AUC), maximum concentration (Cmax), half-life (t1/2), etc.). Pharmacodynamic studies (effects of drugs on target tissues and organs, mechanisms of action, dose-response relationship and determination of therapeutic index, evaluation of side effects of drugs, toxicity studies (acute toxicity, chronic toxicity, genotoxicity, carcinogenicity, reproductive toxicity, teratogenicity)-I, In Vivo Methods (Pharmacokinetic studies (absorption, distribution, metabolism, elimination), pharmacokinetic parameters (area under the curve (AUC), maximum concentration (Cmax), half-life (t1/2) etc.). ) to be determined. Pharmacodynamic studies (effects of drugs on target tissues and organs, mechanisms of action, dose-response relationship and determination of therapeutic index, evaluation of side effects of drugs, toxicity studies (acute toxicity, chronic toxicity, genotoxicity, carcinogenicity, reproductive toxicity, teratogenicity)-II, In Vivo Methods (Pharmacokinetic studies (absorption, distribution, metabolism, elimination), pharmacokinetic parameters (area under the curve (AUC), maximum concentration (Cmax), half-life (t1/2) etc.). ) to be determined. Pharmacodynamic studies (effects of drugs on target tissues and organs, mechanisms of action, dose-response relationship and determination of therapeutic index, evaluation of side effects of drugs, toxicity studies (acute toxicity, chronic toxicity, genotoxicity, carcinogenicity, reproductive toxicity, teratogenicity)-III, Special in vivo models (disease models (e.g. diabetes, cancer, inflammation models), transgenic animal models, knockout animal models), Behavioral tests (motor function tests (open field test, rotarod test), learning and memory tests (maze tests (Y-maze, T-maze), avoidance tests (active and passive avoidance), recognition tests (novel object recognition), anxiety and depression tests (elevated plus maze), dark/light preference test), forced swimming test, tail hanging test), social behavior tests (social interaction test))- I, Special in vivo models (disease models (e.g., diabetes, cancer, inflammation models), transgenic animal models, knockout animal models), behavioral tests (motor function tests (open field test, rotarod test), learning and memory tests (maze tests (Y-labyrinth, T-labyrinth), avoidance tests (active and passive avoidance), recognition tests (novel object recognition), anxiety and depression tests (elevated plus maze), dark/light preference test), forced swim test, tail hanging test), social behavior tests (social interaction test)) - II. , Specialized in vivo models (disease models (e.g. diabetes, cancer, inflammation models), transgenic animal models, knockout animal models), Behavioral tests (motor function tests (open field test, rotarod test), learning and memory tests (maze tests (Y-labyrinth, T-labyrinth), avoidance tests (active and passive avoidance), recognition tests (novel object recognition), anxiety and depression tests (elevated plus maze, dark/light preference test), forced swimming test, tail hanging test), social behavior tests (social interaction test))- III, Techniques used in in vivo studies (blood collection methods, tissue and organ sampling, drug administration methods (oral, intravenous, intraperitoneal, intramuscular, etc.), imaging methods and histopathological examinations, in vivo data analysis and interpretation, advantages and limitations of in vivo studies, future of in vivo studies and new technologies (artificial intelligence, organ-on-a-chip, etc.)

Course Precondition

Not required.

Resources

Scientific database

Notes

Scientific database

Course Learning Outcomes

Relation with Program Learning Outcome

Week Plan

Student Workload - ECTS