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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| BIOTECHNOLOGY (MASTER) (WITH THESIS) (INTERDISCIPLINARY) | |
| Code | BT573 |
| Name | Pesticide Toxicity in Fish and Biotechnological Approaches |
| Term | 2026-2027 Academic Year |
| Term | Spring |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. SELÇUK DUMAN |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
To provide knowledge on toxic effects of pesticide exposure in fish at molecular, biochemical and physiological levels and to develop skills for toxicity evaluation and environmental risk assessment using biotechnological approaches.
Course Content
Acute and chronic toxicity of pesticides in fish, LC50 determination, hematological, biochemical and immunological parameters, stress physiology, biomarker analyses, toxicogenomics, molecular techniques and environmental risk assessment approaches.
Course Precondition
No prerequisites
Resources
Di Giulio, R. T., & Hinton, D. E. (2008). The Toxicology of Fishes. CRC Press.
Notes
Duman, S. (2025). Effects of sublethal concentrations of tribenuron-methyl pesticide on some hematological, immunological and biochemical parameters in Siberian sturgeon (Acipenser baerii), and LC50 value. Polish Journal of Veterinary Sciences, 28(4), 509-518. Walker, C. H., Sibly, R. M., Hopkin, S. P., & Peakall, D. B. (2012). Principles of Ecotoxicology (4th ed.). CRC Press. Rand, G. M. (Ed.). (1995). Fundamentals of Aquatic Toxicology. Taylor & Francis. Di Giulio, R. T., & Hinton, D. E. (2008). The Toxicology of Fishes. CRC Press. Roberts, R. J. (2012). Fish Pathology (4th ed.). Wiley-Blackwell. Current articles indexed in SCI and SCI-Expanded related to the field will be followed within the scope of the course.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explains the toxic effects of pesticides on fish |
| LO02 | Evaluates LC50 and toxicity testing methods |
| LO03 | Interprets hematological and biochemical changes |
| LO04 | Analyzes immune responses and stress indicators |
| LO05 | Explains the use of biomarkers and evaluates toxicogenomic and omics approaches |
| LO06 | Performs environmental risk assessment |
| LO07 | Applies and interprets biotechnological analytical methods |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Based on undergraduate level qualifications, they develop their knowledge at the level of expertise in the same or a different field. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Knows the ethical rules to be considered while obtaining biotechnological products. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | To identifiy the interdisciplinary interaction of the field and to solve use of the methods. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Interpret the knowledge gained in the field by integrating the information from different disciplines and creates new information | 4 |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Develops new strategic approaches for solving complex problems that are encountered in applications related to the field. | 3 |
| PLO06 | Yetkinlikler - Öğrenme Yetkinliği | To teach the social, scientific, cultural and ethical values during the collection, interpretation, implementation and announcement of the data related to the field. | |
| PLO07 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | To solve the problems encountered in the field and cominicate by using research methods. | |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Establishes functional interaction by using strategic decision-making processes in solving the problems encountered in the field. | |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | To gain the ability to develop and deepen the knowledge in the field of biotechnology | 5 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to aquatic toxicology | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 2 | Classification of pesticides and their environmental distribution | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 3 | Mechanisms of toxicity in fish | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 4 | Acute toxicity and LC50 determination methods | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 5 | Chronic toxicity and sublethal effects | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 6 | Hematological parameters and analytical methods | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 7 | Biochemical and stress parameters (glucose, cortisol, etc.) | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 8 | Mid-Term Exam | Reviewing lecture notes and previous topics | Ölçme Yöntemleri: Sözlü Sınav |
| 9 | Immunological responses and cytokines | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 10 | Biomarker concept and applications | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 11 | Molecular biology techniques (PCR, qPCR) | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 12 | Toxicogenomics and gene expression | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 13 | Omics technologies (transcriptomics, proteomics) | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 14 | Environmental risk assessment and modeling | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 15 | Evaluation of current research | Examining primary sources and the suggested article | Öğretim Yöntemleri: Tartışma, Soru-Cevap |
| 16 | Term Exams | Reviewing lecture notes and previous topics | Ölçme Yöntemleri: Sözlü Sınav |
| 17 | Term Exams | Reviewing lecture notes and previous topics | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 10 | 2 | 20 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 2 | 2 |
| Final Exam | 1 | 2 | 2 |
| Total Workload (Hour) | 150 | ||
| Total Workload / 25 (h) | 6,00 | ||
| ECTS | 6 ECTS | ||