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Information
| Unit | INSTITUTE OF NATURAL AND APPLIED SCIENCES |
| ZOOTECHNICS (MASTER) (WITH THESIS) | |
| Code | ZO686 |
| Name | Staining Techniques for Reproductive Cells |
| Term | 2025-2026 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Dr. Öğr. Üyesi Ali Galip ÖNAL |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
To understand the cytological basis of staining techniques for reproductive cells To apply standard and advanced staining protocols for assessing sperm and oocyte quality To interpret stained slides for clinical diagnostics and reproductive research To build laboratory competence in microscopy and sperm evaluation
Course Content
This course provides a comprehensive introduction to classical and modern staining techniques used in the evaluation of sperm and oocyte, embryo and other cells (like granulosa and luteal). It covers the biological significance of each staining method and its application in clinical and research settings. Through a combination of theoretical lectures and hands-on labs, students gain the skills to assess viability, membrane integrity, acrosomal status, DNA fragmentation, and morphology using light and fluorescent microscopy.
Course Precondition
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Resources
Hafez, E.S.E., & Hafez, B. (Reproduction in Farm Animals, 7th or 8th Edition), Gordon, I. (Controlled Reproduction in Cattle and Buffaloes)
Notes
Hafez, E.S.E., & Hafez, B. (Reproduction in Farm Animals, 7th or 8th Edition), Gordon, I. (Controlled Reproduction in Cattle and Buffaloes)
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Describe and identify the morphological features of reproductive cells (sperm and oocytes) and explain standard evaluation criteria. |
| LO02 | Apply classical (e.g., Nigrosine-Eosin, Giemsa) and advanced (e.g., HOST, SYBR-PI, TUNEL) staining techniques to assess reproductive cell quality. |
| LO03 | Report semen evaluation results in a scientific format and perform quality control procedures in the reproductive la |
| LO04 | Analyze stained samples using light and fluorescent microscopy to evaluate viability, morphology, membrane integrity, and DNA fragmentation. |
| LO05 | Develop critical decision-making skills based on sperm quality data for use in assisted reproductive technologies (ART). |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | After undergraduate education, increases knowledge in one of the fields of animal breeding and breeding, feeds and animal nutrition, biometrics and genetics. | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interaction between different disciplines | 4 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains the ability to develop strategic approaches and produce regional, national or international solutions for the field of animal science | 4 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Zootekni bilimindeki verileri kullanarak bilimsel yöntemlerle bilgiyi geliştirebilme, bilimsel, toplumsal ve etik sorumluluk bilinci ile bu bilgileri kullanabilme becerisini kazanır | 3 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Gains the ability to use and develop information technologies with computer software and hardware knowledge required by the field of animal science. | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Gains the ability to convey their own studies or current developments in the field of animal science to groups in the field or other fields of science, verbally and visually. | 3 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Gains the ability to evaluate the quality processes of animal products | |
| PLO08 | Bilgi - Kuramsal, Olgusal | Gains the ability to keep animal production dynamic in accordance with changing economic and social conditions. | |
| PLO09 | Bilgi - Kuramsal, Olgusal | Gains the ability to follow national and international current issues, to follow developments in lifelong learning, science and technology, to constantly renew themselves and to transfer innovations to animal production. | 3 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Absorbs the relationship between animal products and human health and community welfare |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Overview of sperm and oocyte morphology; principles of cytological staining | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 2 | Semen analysis protocols, Sperm preparation, concentration & motility evaluation | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 3 | Nigrosine-Eosin principles and interpretation | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 4 | Staining methods with PNA-FITC, CYBER, PI/DAPI and SYBR-14 | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 5 | Staining methods with PNA-FITC, CYBER, PI/DAPI and SYBR-14 II | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 6 | Performing Hypo-Osmotic Swelling Test (HOST) | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 7 | Morphology analysis using Diff-Quick/Giemsa | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 8 | Mid-Term Exam | Course References | Ölçme Yöntemleri: Ödev |
| 9 | Oocyte viability staining (Trypan Blue, Hoechst) | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 10 | Dual staining using SYBR-14 and Propidium Iodide | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 11 | Annexin-V/PI staining protocol and analysis | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 12 | Microscopy skills, CASA system, image analysis | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 13 | Sperm acrosome staining | Course References | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 14 | Course wrap-up | Course References | Öğretim Yöntemleri: Soru-Cevap |
| 15 | Course wrap-up II | Course References | Öğretim Yöntemleri: Soru-Cevap |
| 16 | Term Exams | Course References | Ölçme Yöntemleri: Ödev |
| 17 | Term Exams | Course References | Ölçme Yöntemleri: Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 1 | 40 | 40 |
| Out of Class Study (Preliminary Work, Practice) | 1 | 30 | 30 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 30 | 30 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 30 | 30 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 160 | ||
| Total Workload / 25 (h) | 6,40 | ||
| ECTS | 6 ECTS | ||